We reproduce chemical reactions of blood vessels by a flexibly deformable collagen tube device. The device is composed of an unfixed collagen hydrogel tube, enabling the reproduction of both macro and micro scale deformation of the vessel tissue. In addition, the device is easily perfused because the collagen hydrogel tube is directly attached to silicone tubes. We succeeded in demonstrating the chemical deformation of vessel tissue and endothelial cells by inflammatory mediators. Moreover, the drug testing was presented by anti-inflammatory chemicals. We believe that our flexible culturing device could help the construction of functional blood vessels reproducing in vivo chemical reactions in high similarity, and contribute to biomedical researches and pharmacokinetic testing.