Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation

Kenji Kanazawa, Seinosuke Kawashima, Shuji Mikami, Yoichi Miwa, Ken Ichi Hirata, Masakuni Suematsu, Yoshitake Hayashi, Hiroshi Itoh, Mitsuhiro Yokoyama

研究成果: Article

90 引用 (Scopus)

抄録

Atherosclerotic arteries are well known to exhibit impaired endothelium-dependent relaxation (EDR), but the exact mechanism of this impairment remains unclear. Recently, endothelial constitutive nitric oxide synthase (ECNOS), which generates nitric oxide and mediates EDR, was cloned, and ECNOS mRNA expression was reported to be modified by various cytokines, lipoproteins, and shear stress. To investigate the expression of ECNOS mRNA and protein in atherosclerotic arteries with impaired EDR, thoracic aortas isolated from Watanabe heritable hyperlipidemic (WHHL) rabbits were examined by using in situ hybridization and immunohistochemistry. Compared with thoracic aortas from Japanese White rabbits, WaHL aortas exhibited significantly impaired EDRs, although both in situ hybridization and immunohistochemistry exhibited enhanced expression of ECNOS mRNA and protein in WHHL aortas. There was no significant relationship between expression of ECNOS mRNA and protein of endothelial cells and age of the examined WHHL aortas. These data suggest that the mechanism of impaired EDR in atherosclerotic arteries is not due to the decrease in ECNOS mRNA and protein.

元の言語English
ページ(範囲)1949-1956
ページ数8
ジャーナルAmerican Journal of Pathology
148
発行部数6
出版物ステータスPublished - 1996 6
外部発表Yes

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Nitric Oxide Synthase Type III
Vasodilation
Aorta
Rabbits
Messenger RNA
Endothelium
Proteins
Arteries
Thoracic Aorta
In Situ Hybridization
Immunohistochemistry
Lipoproteins
Nitric Oxide
Endothelial Cells
Cytokines

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

これを引用

Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation. / Kanazawa, Kenji; Kawashima, Seinosuke; Mikami, Shuji; Miwa, Yoichi; Hirata, Ken Ichi; Suematsu, Masakuni; Hayashi, Yoshitake; Itoh, Hiroshi; Yokoyama, Mitsuhiro.

:: American Journal of Pathology, 巻 148, 番号 6, 06.1996, p. 1949-1956.

研究成果: Article

Kanazawa, K, Kawashima, S, Mikami, S, Miwa, Y, Hirata, KI, Suematsu, M, Hayashi, Y, Itoh, H & Yokoyama, M 1996, 'Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation', American Journal of Pathology, 巻. 148, 番号 6, pp. 1949-1956.
Kanazawa, Kenji ; Kawashima, Seinosuke ; Mikami, Shuji ; Miwa, Yoichi ; Hirata, Ken Ichi ; Suematsu, Masakuni ; Hayashi, Yoshitake ; Itoh, Hiroshi ; Yokoyama, Mitsuhiro. / Endothelial constitutive nitric oxide synthase protein and mRNA increased in rabbit atherosclerotic aorta despite impaired endothelium-dependent vascular relaxation. :: American Journal of Pathology. 1996 ; 巻 148, 番号 6. pp. 1949-1956.
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abstract = "Atherosclerotic arteries are well known to exhibit impaired endothelium-dependent relaxation (EDR), but the exact mechanism of this impairment remains unclear. Recently, endothelial constitutive nitric oxide synthase (ECNOS), which generates nitric oxide and mediates EDR, was cloned, and ECNOS mRNA expression was reported to be modified by various cytokines, lipoproteins, and shear stress. To investigate the expression of ECNOS mRNA and protein in atherosclerotic arteries with impaired EDR, thoracic aortas isolated from Watanabe heritable hyperlipidemic (WHHL) rabbits were examined by using in situ hybridization and immunohistochemistry. Compared with thoracic aortas from Japanese White rabbits, WaHL aortas exhibited significantly impaired EDRs, although both in situ hybridization and immunohistochemistry exhibited enhanced expression of ECNOS mRNA and protein in WHHL aortas. There was no significant relationship between expression of ECNOS mRNA and protein of endothelial cells and age of the examined WHHL aortas. These data suggest that the mechanism of impaired EDR in atherosclerotic arteries is not due to the decrease in ECNOS mRNA and protein.",
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AU - Kawashima, Seinosuke

AU - Mikami, Shuji

AU - Miwa, Yoichi

AU - Hirata, Ken Ichi

AU - Suematsu, Masakuni

AU - Hayashi, Yoshitake

AU - Itoh, Hiroshi

AU - Yokoyama, Mitsuhiro

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N2 - Atherosclerotic arteries are well known to exhibit impaired endothelium-dependent relaxation (EDR), but the exact mechanism of this impairment remains unclear. Recently, endothelial constitutive nitric oxide synthase (ECNOS), which generates nitric oxide and mediates EDR, was cloned, and ECNOS mRNA expression was reported to be modified by various cytokines, lipoproteins, and shear stress. To investigate the expression of ECNOS mRNA and protein in atherosclerotic arteries with impaired EDR, thoracic aortas isolated from Watanabe heritable hyperlipidemic (WHHL) rabbits were examined by using in situ hybridization and immunohistochemistry. Compared with thoracic aortas from Japanese White rabbits, WaHL aortas exhibited significantly impaired EDRs, although both in situ hybridization and immunohistochemistry exhibited enhanced expression of ECNOS mRNA and protein in WHHL aortas. There was no significant relationship between expression of ECNOS mRNA and protein of endothelial cells and age of the examined WHHL aortas. These data suggest that the mechanism of impaired EDR in atherosclerotic arteries is not due to the decrease in ECNOS mRNA and protein.

AB - Atherosclerotic arteries are well known to exhibit impaired endothelium-dependent relaxation (EDR), but the exact mechanism of this impairment remains unclear. Recently, endothelial constitutive nitric oxide synthase (ECNOS), which generates nitric oxide and mediates EDR, was cloned, and ECNOS mRNA expression was reported to be modified by various cytokines, lipoproteins, and shear stress. To investigate the expression of ECNOS mRNA and protein in atherosclerotic arteries with impaired EDR, thoracic aortas isolated from Watanabe heritable hyperlipidemic (WHHL) rabbits were examined by using in situ hybridization and immunohistochemistry. Compared with thoracic aortas from Japanese White rabbits, WaHL aortas exhibited significantly impaired EDRs, although both in situ hybridization and immunohistochemistry exhibited enhanced expression of ECNOS mRNA and protein in WHHL aortas. There was no significant relationship between expression of ECNOS mRNA and protein of endothelial cells and age of the examined WHHL aortas. These data suggest that the mechanism of impaired EDR in atherosclerotic arteries is not due to the decrease in ECNOS mRNA and protein.

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