Endothelin in human plasma and culture medium of aortic endothelial cells-Detection and characterization with radioimmunoassay using monoclonal antibody

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Itoh, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Gotaro Shirakami, Shin ichi Suga, Michihisa Jougasaki, Shoko Morichika, Hiroo Imura

研究成果: Article査読

82 被引用数 (Scopus)

抄録

We have developed monoclonal (KY-ET-1-I) and polyclonal (ET-F5) antibodies against endothelin-1 (ET-1) and established sensitive radioimmunoassays (RIAs) with different specificities. The RIA with KY-ET-1-I detected ET-1, ET-2 and ET-3, while the RIA with ET-F5 recognized ET-3 very weakly. Using these RIAs, we have investigated the concentration and molecular forms of ET-1-like immunoreactivity (-LI) in culture medium of bovine aortic endothelial cells and human plasma. Culture medium of endothelial cells contained two major components compatible with big ET and ET-1. ET-1-LI was also detected in human plasma. ET-1-LI in human plasma consisted of apparent two components, the small molecular form emerging at the position of ET-1 and the large form with the peak eluting at the preceding fraction of the elution position of big ET. The concentration of the small form of ET in human plasma was about 5 pg/ml.

本文言語English
ページ(範囲)320-326
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
161
1
DOI
出版ステータスPublished - 1989 5 30
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Endothelin in human plasma and culture medium of aortic endothelial cells-Detection and characterization with radioimmunoassay using monoclonal antibody」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル