Enhanced angiogenic potency of monocytic endothelial progenitor cells in patients with systemic sclerosis

Yukie Yamaguchi, Yuka Okazaki, Noriyuki Seta, Takashi Satoh, Kazuo Takahashi, Zenro Ikezawa, Masataka Kuwana

研究成果: Article

22 引用 (Scopus)

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Introduction: Microvasculopathy is one of the characteristic features in patients with systemic sclerosis (SSc), but underlying mechanisms still remain uncertain. In this study, we evaluated the potential involvement of monocytic endothelial progenitor cells (EPCs) in pathogenic processes of SSc vasculopathy, by determining their number and contribution to blood vessel formation through angiogenesis and vasculogenesis.Methods: Monocytic EPCs were enriched and enumerated using a culture of peripheral blood mononuclear cells and platelets on fibronectin in 23 patients with SSc, 22 patients with rheumatoid arthritis (RA), and 21 healthy controls. To assess the capacity of monocytic EPCs to promote vascular formation and the contribution of vasculogenesis to this process, we used an in vitro co-culture system with human umbilical vein endothelial cells (HUVECs) on Matrigel® and an in vivo murine tumor neovascularization model.Results: Monocytic EPCs were significantly increased in SSc patients than in RA patients or healthy controls (P = 0.01 for both comparisons). Monocytic EPCs derived from SSc patients promoted tubular formation in Matrigel® cultures more than those from healthy controls (P = 0.007). Transplantation of monocytic EPCs into immunodeficient mice resulted in promotion of tumor growth and blood vessel formation, and these properties were more prominent in SSc than healthy monocytic EPCs (P = 0.03 for both comparisons). In contrast, incorporation of SSc monocytic EPCs into the tubular structure was less efficient in vitro and in vivo, compared with healthy monocytic EPCs.Conclusions: SSc patients have high numbers of aberrant circulating monocytic EPCs that exert enhanced angiogenesis but are impaired in vasculogenesis. However, these cells apparently cannot overcome the anti-angiogenic environment that characterizes SSc-affected tissues.

元の言語English
記事番号R205
ジャーナルArthritis Research and Therapy
12
発行部数6
DOI
出版物ステータスPublished - 2010 11 4

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ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

これを引用

Yamaguchi, Y., Okazaki, Y., Seta, N., Satoh, T., Takahashi, K., Ikezawa, Z., & Kuwana, M. (2010). Enhanced angiogenic potency of monocytic endothelial progenitor cells in patients with systemic sclerosis. Arthritis Research and Therapy, 12(6), [R205]. https://doi.org/10.1186/ar3180