Enhanced bacterial efflux system is the first step to the development of metronidazole resistance in Helicobacter pylori

Hitoshi Tsugawa, Hidekazu Suzuki, Hiroe Muraoka, Fumiaki Ikeda, Kenro Hirata, Juntaro Matsuzaki, Yoshimasa Saito, Toshifumi Hibi

研究成果: Article

28 引用 (Scopus)

抄録

Although metronidazole (Mtz) is an important component of Helicobacter pylori eradication regimens, it has been pointed out that the increasing use of Mtz may result in increase in the incidence of Mtz-resistant strains. The present study was designed to examine the initial mechanism of resistance acquisition of H. pylori to Mtz. After 10 Mtz-susceptible strains were cultured on plates containing sub-inhibitory concentrations of Mtz, the MIC of Mtz for 9 of the 10 strains increased to levels of the Mtz-resistant strains. In the Mtz-resistance-induced strains, the expression of the TolC efflux pump (hefA) was significantly increased under Mtz exposure, without the reduction of the Mtz-reductive activity. Our finding suggests that overexpression of hefA may be the initial step in the acquisition of Mtz resistance in H. pylori.

元の言語English
ページ(範囲)656-660
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
404
発行部数2
DOI
出版物ステータスPublished - 2011 1 14

Fingerprint

Metronidazole
Helicobacter pylori
Pumps

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

これを引用

@article{a820aa3564964c04a3465f6856786067,
title = "Enhanced bacterial efflux system is the first step to the development of metronidazole resistance in Helicobacter pylori",
abstract = "Although metronidazole (Mtz) is an important component of Helicobacter pylori eradication regimens, it has been pointed out that the increasing use of Mtz may result in increase in the incidence of Mtz-resistant strains. The present study was designed to examine the initial mechanism of resistance acquisition of H. pylori to Mtz. After 10 Mtz-susceptible strains were cultured on plates containing sub-inhibitory concentrations of Mtz, the MIC of Mtz for 9 of the 10 strains increased to levels of the Mtz-resistant strains. In the Mtz-resistance-induced strains, the expression of the TolC efflux pump (hefA) was significantly increased under Mtz exposure, without the reduction of the Mtz-reductive activity. Our finding suggests that overexpression of hefA may be the initial step in the acquisition of Mtz resistance in H. pylori.",
keywords = "Antimicrobial agent, Drug resistance, Efflux pump, HefA, RdxA",
author = "Hitoshi Tsugawa and Hidekazu Suzuki and Hiroe Muraoka and Fumiaki Ikeda and Kenro Hirata and Juntaro Matsuzaki and Yoshimasa Saito and Toshifumi Hibi",
year = "2011",
month = "1",
day = "14",
doi = "10.1016/j.bbrc.2010.12.034",
language = "English",
volume = "404",
pages = "656--660",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Enhanced bacterial efflux system is the first step to the development of metronidazole resistance in Helicobacter pylori

AU - Tsugawa, Hitoshi

AU - Suzuki, Hidekazu

AU - Muraoka, Hiroe

AU - Ikeda, Fumiaki

AU - Hirata, Kenro

AU - Matsuzaki, Juntaro

AU - Saito, Yoshimasa

AU - Hibi, Toshifumi

PY - 2011/1/14

Y1 - 2011/1/14

N2 - Although metronidazole (Mtz) is an important component of Helicobacter pylori eradication regimens, it has been pointed out that the increasing use of Mtz may result in increase in the incidence of Mtz-resistant strains. The present study was designed to examine the initial mechanism of resistance acquisition of H. pylori to Mtz. After 10 Mtz-susceptible strains were cultured on plates containing sub-inhibitory concentrations of Mtz, the MIC of Mtz for 9 of the 10 strains increased to levels of the Mtz-resistant strains. In the Mtz-resistance-induced strains, the expression of the TolC efflux pump (hefA) was significantly increased under Mtz exposure, without the reduction of the Mtz-reductive activity. Our finding suggests that overexpression of hefA may be the initial step in the acquisition of Mtz resistance in H. pylori.

AB - Although metronidazole (Mtz) is an important component of Helicobacter pylori eradication regimens, it has been pointed out that the increasing use of Mtz may result in increase in the incidence of Mtz-resistant strains. The present study was designed to examine the initial mechanism of resistance acquisition of H. pylori to Mtz. After 10 Mtz-susceptible strains were cultured on plates containing sub-inhibitory concentrations of Mtz, the MIC of Mtz for 9 of the 10 strains increased to levels of the Mtz-resistant strains. In the Mtz-resistance-induced strains, the expression of the TolC efflux pump (hefA) was significantly increased under Mtz exposure, without the reduction of the Mtz-reductive activity. Our finding suggests that overexpression of hefA may be the initial step in the acquisition of Mtz resistance in H. pylori.

KW - Antimicrobial agent

KW - Drug resistance

KW - Efflux pump

KW - HefA

KW - RdxA

UR - http://www.scopus.com/inward/record.url?scp=78651371647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78651371647&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2010.12.034

DO - 10.1016/j.bbrc.2010.12.034

M3 - Article

C2 - 21147064

AN - SCOPUS:78651371647

VL - 404

SP - 656

EP - 660

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -