Enhanced Fluorescence Using an Optical Interference Mirror Overlaid with Silver Island Film

Takafumi Harashima, Mitsuru Yasuda, Takuo Akimoto

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Fluorophores overlaid on an optical interference mirror composed of a metal and thin dielectric layer demonstrate enhanced fluorescence. Fluorescence is also enhanced by silver nanostructures such as silver island films, which excite localized surface plasmon resonance. An optical interference mirror surface was overlaid with a silver island film to amplify the fluorescence enhancement. The optimal thickness of the silver island film (100 nm) was evaluated from transmittance and surface roughness measurements. At this thickness, the fluorescence was amplified sixteen-fold. The thickness of the interference layer was optimized at 40 nm providing a one hundred-sixty fold fluorescence enhancement of rhodamine B. However, only a four-fold improvement in sensitivity was achieved for the determination of a labeled streptavidin using biotin immobilized on the silver island film interference mirror.

本文言語English
ページ(範囲)2731-2739
ページ数9
ジャーナルAnalytical Letters
47
16
DOI
出版ステータスPublished - 2014 11月 1
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分光学
  • 臨床生化学
  • 生化学、医学
  • 電気化学

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