Enhancement of optical sectioning capability of temporal focusing microscopy by using time-multiplexed multi-line focusing

Kenta Inazawa, Keisuke Isobe, Tomohiro Ishikawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Temporal focusing (TF) microscopy has a trade-off between temporal resolution, optical sectioning capability, and field of view. To improve this trade-off, we demonstrate time-multiplexed multi-line temporal focusing (TM-ML-TF) microscopy, which combines the time-multiplexed TF technique with multi-line focusing using a digital micromirror device. Compared with those for TF microscopy, the optical sectioning capability and the signal-to-background ratio were improved by factors of 2.5 and 4.17, respectively. In addition, by applying post-processing for structured illumination microscopy to TM-ML-TF microscopy, the optical sectioning capability and the signal-to-background ratio were further enhanced by factors of 1.2 and 4.63, respectively.

本文言語English
論文番号082008
ジャーナルApplied Physics Express
14
8
DOI
出版ステータスPublished - 2021 8月

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(全般)

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