Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits

Kiyoshi Moriyama, Akitoshi Ishizaka, Morio Nakamura, Hiroshi Kubo, Toru Kotani, Shinichi Yamamoto, Eileen N. Ogawa, Osamu Kajikawa, Charles W. Frevert, Yoshifumi Kotake, Hiroshi Morisaki, Hidefumi Koh, Sadatomo Tasaka, Thomas R. Martin, Junzo Takeda

研究成果: Article

40 引用 (Scopus)

抄録

Ventilation with a small tidal volume (Vt) is associated with better clinical outcomes than with a large Vt particularly in critical settings, including acute lung injury. To determine whether V t influences the lipopolysaccaharide (LPS) recognition pathway, we studied CD 14 expression in rabbit lungs and the release of TNF-α by cultured alveolar macrophages after 240 min of ventilation with a large (20 ml/kg) vs. a small (5 ml/kg) Vt. We also applied small or large Vt to lungs instilled with 50 μg/kg of LPS. The alveolar macrophages collected after large Vt ventilation revealed a 20-fold increase in LPS-induced TNF-α release compared with those collected after small Vt ventilation, whereas TNF-α was undetectable without LPS stimulation. In animals ventilated with a large Vt, the expression of CD14 mRNA in whole lung homogenates and the expression of CD14 protein on alveolar macrophages, assessed by immunohistochemistry, were both significantly increased in the absence of LPS stimulation. A large V t applied to LPS-instilled lungs increased the pulmonary albumin permeability and TNF-α release into the plasma. These results suggest that mechanical stress caused by a large Vt sensitizes the lungs to endotoxin, a phenomenon that may occur partially via the upregulation of CD14.

元の言語English
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
286
発行部数6 30-6
DOI
出版物ステータスPublished - 2004 6

Fingerprint

Tidal Volume
Endotoxins
Ventilation
Rabbits
Lung
Alveolar Macrophages
Mechanical Stress
Acute Lung Injury
Albumins
Permeability
Up-Regulation
Immunohistochemistry
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

これを引用

Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits. / Moriyama, Kiyoshi; Ishizaka, Akitoshi; Nakamura, Morio; Kubo, Hiroshi; Kotani, Toru; Yamamoto, Shinichi; Ogawa, Eileen N.; Kajikawa, Osamu; Frevert, Charles W.; Kotake, Yoshifumi; Morisaki, Hiroshi; Koh, Hidefumi; Tasaka, Sadatomo; Martin, Thomas R.; Takeda, Junzo.

:: American Journal of Physiology - Lung Cellular and Molecular Physiology, 巻 286, 番号 6 30-6, 06.2004.

研究成果: Article

Moriyama, K, Ishizaka, A, Nakamura, M, Kubo, H, Kotani, T, Yamamoto, S, Ogawa, EN, Kajikawa, O, Frevert, CW, Kotake, Y, Morisaki, H, Koh, H, Tasaka, S, Martin, TR & Takeda, J 2004, 'Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits', American Journal of Physiology - Lung Cellular and Molecular Physiology, 巻. 286, 番号 6 30-6. https://doi.org/10.1152/ajplung.00296.2003
Moriyama, Kiyoshi ; Ishizaka, Akitoshi ; Nakamura, Morio ; Kubo, Hiroshi ; Kotani, Toru ; Yamamoto, Shinichi ; Ogawa, Eileen N. ; Kajikawa, Osamu ; Frevert, Charles W. ; Kotake, Yoshifumi ; Morisaki, Hiroshi ; Koh, Hidefumi ; Tasaka, Sadatomo ; Martin, Thomas R. ; Takeda, Junzo. / Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits. :: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2004 ; 巻 286, 番号 6 30-6.
@article{3b416af67954434388a21bd9ee2b63b0,
title = "Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits",
abstract = "Ventilation with a small tidal volume (Vt) is associated with better clinical outcomes than with a large Vt particularly in critical settings, including acute lung injury. To determine whether V t influences the lipopolysaccaharide (LPS) recognition pathway, we studied CD 14 expression in rabbit lungs and the release of TNF-α by cultured alveolar macrophages after 240 min of ventilation with a large (20 ml/kg) vs. a small (5 ml/kg) Vt. We also applied small or large Vt to lungs instilled with 50 μg/kg of LPS. The alveolar macrophages collected after large Vt ventilation revealed a 20-fold increase in LPS-induced TNF-α release compared with those collected after small Vt ventilation, whereas TNF-α was undetectable without LPS stimulation. In animals ventilated with a large Vt, the expression of CD14 mRNA in whole lung homogenates and the expression of CD14 protein on alveolar macrophages, assessed by immunohistochemistry, were both significantly increased in the absence of LPS stimulation. A large V t applied to LPS-instilled lungs increased the pulmonary albumin permeability and TNF-α release into the plasma. These results suggest that mechanical stress caused by a large Vt sensitizes the lungs to endotoxin, a phenomenon that may occur partially via the upregulation of CD14.",
keywords = "Acute lung injury, Ventilator-induced lung injury",
author = "Kiyoshi Moriyama and Akitoshi Ishizaka and Morio Nakamura and Hiroshi Kubo and Toru Kotani and Shinichi Yamamoto and Ogawa, {Eileen N.} and Osamu Kajikawa and Frevert, {Charles W.} and Yoshifumi Kotake and Hiroshi Morisaki and Hidefumi Koh and Sadatomo Tasaka and Martin, {Thomas R.} and Junzo Takeda",
year = "2004",
month = "6",
doi = "10.1152/ajplung.00296.2003",
language = "English",
volume = "286",
journal = "American Journal of Physiology - Lung Cellular and Molecular Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "6 30-6",

}

TY - JOUR

T1 - Enhancement of the endotoxin recognition pathway by ventilation with a large tidal volume in rabbits

AU - Moriyama, Kiyoshi

AU - Ishizaka, Akitoshi

AU - Nakamura, Morio

AU - Kubo, Hiroshi

AU - Kotani, Toru

AU - Yamamoto, Shinichi

AU - Ogawa, Eileen N.

AU - Kajikawa, Osamu

AU - Frevert, Charles W.

AU - Kotake, Yoshifumi

AU - Morisaki, Hiroshi

AU - Koh, Hidefumi

AU - Tasaka, Sadatomo

AU - Martin, Thomas R.

AU - Takeda, Junzo

PY - 2004/6

Y1 - 2004/6

N2 - Ventilation with a small tidal volume (Vt) is associated with better clinical outcomes than with a large Vt particularly in critical settings, including acute lung injury. To determine whether V t influences the lipopolysaccaharide (LPS) recognition pathway, we studied CD 14 expression in rabbit lungs and the release of TNF-α by cultured alveolar macrophages after 240 min of ventilation with a large (20 ml/kg) vs. a small (5 ml/kg) Vt. We also applied small or large Vt to lungs instilled with 50 μg/kg of LPS. The alveolar macrophages collected after large Vt ventilation revealed a 20-fold increase in LPS-induced TNF-α release compared with those collected after small Vt ventilation, whereas TNF-α was undetectable without LPS stimulation. In animals ventilated with a large Vt, the expression of CD14 mRNA in whole lung homogenates and the expression of CD14 protein on alveolar macrophages, assessed by immunohistochemistry, were both significantly increased in the absence of LPS stimulation. A large V t applied to LPS-instilled lungs increased the pulmonary albumin permeability and TNF-α release into the plasma. These results suggest that mechanical stress caused by a large Vt sensitizes the lungs to endotoxin, a phenomenon that may occur partially via the upregulation of CD14.

AB - Ventilation with a small tidal volume (Vt) is associated with better clinical outcomes than with a large Vt particularly in critical settings, including acute lung injury. To determine whether V t influences the lipopolysaccaharide (LPS) recognition pathway, we studied CD 14 expression in rabbit lungs and the release of TNF-α by cultured alveolar macrophages after 240 min of ventilation with a large (20 ml/kg) vs. a small (5 ml/kg) Vt. We also applied small or large Vt to lungs instilled with 50 μg/kg of LPS. The alveolar macrophages collected after large Vt ventilation revealed a 20-fold increase in LPS-induced TNF-α release compared with those collected after small Vt ventilation, whereas TNF-α was undetectable without LPS stimulation. In animals ventilated with a large Vt, the expression of CD14 mRNA in whole lung homogenates and the expression of CD14 protein on alveolar macrophages, assessed by immunohistochemistry, were both significantly increased in the absence of LPS stimulation. A large V t applied to LPS-instilled lungs increased the pulmonary albumin permeability and TNF-α release into the plasma. These results suggest that mechanical stress caused by a large Vt sensitizes the lungs to endotoxin, a phenomenon that may occur partially via the upregulation of CD14.

KW - Acute lung injury

KW - Ventilator-induced lung injury

UR - http://www.scopus.com/inward/record.url?scp=2442694185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442694185&partnerID=8YFLogxK

U2 - 10.1152/ajplung.00296.2003

DO - 10.1152/ajplung.00296.2003

M3 - Article

C2 - 14633514

AN - SCOPUS:2442694185

VL - 286

JO - American Journal of Physiology - Lung Cellular and Molecular Physiology

JF - American Journal of Physiology - Lung Cellular and Molecular Physiology

SN - 1040-0605

IS - 6 30-6

ER -