Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan

Hiroshi Tachibana, Xun Jia Cheng, Seiki Kobayashi, Yukitoki Fujita, Toshifumi Udono

研究成果: Article

23 引用 (Scopus)

抄録

Chimpanzees (Pan troglodytes) residing in the Kumamoto Primate Research Park, Sanwa Kagaku Kenkyusho, were surveyed for the presence of intestinal parasites. Stool samples from 107 chimpanzees were examined by microscopy after formalin-ether sedimentation. Of these animals, 100 were infected with at least 1 species of ameba. The positivity rates recorded were as follows: Entamoeba coli, 88%; E. histolytica/E. dispar, 48%; E. hartmanni, 15%; Iodamoeba buetschlii, 8%; Endolimax nana, 4%; and Entamoeba chattoni, 2%. Polymerase chain reaction (PCR) analysis to distinguish between E. histolytica and E. dispar was performed on these samples. E. dispar DNA was detected in 60 of 107 samples (56%), including 9 that had been microscopically determined to be negative for E. histolytical E. dispar. In contrast, no E. histolytica DNA was detected in the 107 samples. Zymodeme analysis indicated that 10 isolates were E. dispar. When 104 chimpanzees were examined serologically for E. histolytica infection, 1 sample was scored as positive by indirect hemagglutination and another was found to be positive by an indirect fluorescent antibody test. However, both specimens were borderline-positive and were clearly negative in other tests, suggesting that they might be false-positives. These results demonstrate that the pathogenic E. histolytica was absent in this colony, regardless of the high degree of prevalence of other amebas. For an accurate diagnosis, PCR analysis is recommended in addition to microscopic examination.

元の言語English
ページ(範囲)537-541
ページ数5
ジャーナルParasitology Research
86
発行部数7
出版物ステータスPublished - 2000

Fingerprint

Entamoeba
Entamoeba histolytica
Pan troglodytes
Japan
Amoeba
Endolimax
sampling
Polymerase Chain Reaction
polymerase chain reaction
DNA
Hemagglutination
Ether
Primates
Formaldehyde
Microscopy
hemagglutination
Parasites
formalin
ethers
microscopy

ASJC Scopus subject areas

  • Parasitology

これを引用

Tachibana, H., Cheng, X. J., Kobayashi, S., Fujita, Y., & Udono, T. (2000). Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan. Parasitology Research, 86(7), 537-541.

Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan. / Tachibana, Hiroshi; Cheng, Xun Jia; Kobayashi, Seiki; Fujita, Yukitoki; Udono, Toshifumi.

:: Parasitology Research, 巻 86, 番号 7, 2000, p. 537-541.

研究成果: Article

Tachibana, H, Cheng, XJ, Kobayashi, S, Fujita, Y & Udono, T 2000, 'Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan', Parasitology Research, 巻. 86, 番号 7, pp. 537-541.
Tachibana H, Cheng XJ, Kobayashi S, Fujita Y, Udono T. Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan. Parasitology Research. 2000;86(7):537-541.
Tachibana, Hiroshi ; Cheng, Xun Jia ; Kobayashi, Seiki ; Fujita, Yukitoki ; Udono, Toshifumi. / Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan. :: Parasitology Research. 2000 ; 巻 86, 番号 7. pp. 537-541.
@article{907538ce766c47d88f74a062ce6f51dd,
title = "Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan",
abstract = "Chimpanzees (Pan troglodytes) residing in the Kumamoto Primate Research Park, Sanwa Kagaku Kenkyusho, were surveyed for the presence of intestinal parasites. Stool samples from 107 chimpanzees were examined by microscopy after formalin-ether sedimentation. Of these animals, 100 were infected with at least 1 species of ameba. The positivity rates recorded were as follows: Entamoeba coli, 88{\%}; E. histolytica/E. dispar, 48{\%}; E. hartmanni, 15{\%}; Iodamoeba buetschlii, 8{\%}; Endolimax nana, 4{\%}; and Entamoeba chattoni, 2{\%}. Polymerase chain reaction (PCR) analysis to distinguish between E. histolytica and E. dispar was performed on these samples. E. dispar DNA was detected in 60 of 107 samples (56{\%}), including 9 that had been microscopically determined to be negative for E. histolytical E. dispar. In contrast, no E. histolytica DNA was detected in the 107 samples. Zymodeme analysis indicated that 10 isolates were E. dispar. When 104 chimpanzees were examined serologically for E. histolytica infection, 1 sample was scored as positive by indirect hemagglutination and another was found to be positive by an indirect fluorescent antibody test. However, both specimens were borderline-positive and were clearly negative in other tests, suggesting that they might be false-positives. These results demonstrate that the pathogenic E. histolytica was absent in this colony, regardless of the high degree of prevalence of other amebas. For an accurate diagnosis, PCR analysis is recommended in addition to microscopic examination.",
author = "Hiroshi Tachibana and Cheng, {Xun Jia} and Seiki Kobayashi and Yukitoki Fujita and Toshifumi Udono",
year = "2000",
language = "English",
volume = "86",
pages = "537--541",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0044-3255",
publisher = "Springer Verlag",
number = "7",

}

TY - JOUR

T1 - Entamoeba dispar, but not E. histolytica, detected in a colony of chimpanzees in Japan

AU - Tachibana, Hiroshi

AU - Cheng, Xun Jia

AU - Kobayashi, Seiki

AU - Fujita, Yukitoki

AU - Udono, Toshifumi

PY - 2000

Y1 - 2000

N2 - Chimpanzees (Pan troglodytes) residing in the Kumamoto Primate Research Park, Sanwa Kagaku Kenkyusho, were surveyed for the presence of intestinal parasites. Stool samples from 107 chimpanzees were examined by microscopy after formalin-ether sedimentation. Of these animals, 100 were infected with at least 1 species of ameba. The positivity rates recorded were as follows: Entamoeba coli, 88%; E. histolytica/E. dispar, 48%; E. hartmanni, 15%; Iodamoeba buetschlii, 8%; Endolimax nana, 4%; and Entamoeba chattoni, 2%. Polymerase chain reaction (PCR) analysis to distinguish between E. histolytica and E. dispar was performed on these samples. E. dispar DNA was detected in 60 of 107 samples (56%), including 9 that had been microscopically determined to be negative for E. histolytical E. dispar. In contrast, no E. histolytica DNA was detected in the 107 samples. Zymodeme analysis indicated that 10 isolates were E. dispar. When 104 chimpanzees were examined serologically for E. histolytica infection, 1 sample was scored as positive by indirect hemagglutination and another was found to be positive by an indirect fluorescent antibody test. However, both specimens were borderline-positive and were clearly negative in other tests, suggesting that they might be false-positives. These results demonstrate that the pathogenic E. histolytica was absent in this colony, regardless of the high degree of prevalence of other amebas. For an accurate diagnosis, PCR analysis is recommended in addition to microscopic examination.

AB - Chimpanzees (Pan troglodytes) residing in the Kumamoto Primate Research Park, Sanwa Kagaku Kenkyusho, were surveyed for the presence of intestinal parasites. Stool samples from 107 chimpanzees were examined by microscopy after formalin-ether sedimentation. Of these animals, 100 were infected with at least 1 species of ameba. The positivity rates recorded were as follows: Entamoeba coli, 88%; E. histolytica/E. dispar, 48%; E. hartmanni, 15%; Iodamoeba buetschlii, 8%; Endolimax nana, 4%; and Entamoeba chattoni, 2%. Polymerase chain reaction (PCR) analysis to distinguish between E. histolytica and E. dispar was performed on these samples. E. dispar DNA was detected in 60 of 107 samples (56%), including 9 that had been microscopically determined to be negative for E. histolytical E. dispar. In contrast, no E. histolytica DNA was detected in the 107 samples. Zymodeme analysis indicated that 10 isolates were E. dispar. When 104 chimpanzees were examined serologically for E. histolytica infection, 1 sample was scored as positive by indirect hemagglutination and another was found to be positive by an indirect fluorescent antibody test. However, both specimens were borderline-positive and were clearly negative in other tests, suggesting that they might be false-positives. These results demonstrate that the pathogenic E. histolytica was absent in this colony, regardless of the high degree of prevalence of other amebas. For an accurate diagnosis, PCR analysis is recommended in addition to microscopic examination.

UR - http://www.scopus.com/inward/record.url?scp=0033826603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033826603&partnerID=8YFLogxK

M3 - Article

C2 - 10935902

AN - SCOPUS:0033826603

VL - 86

SP - 537

EP - 541

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0044-3255

IS - 7

ER -