TY - JOUR
T1 - Entamoeba histolytica
T2 - Inhibition in vitro by bithionol of respiratory activity and growth
AU - Takeuchi, Tsutomu
AU - Kobayashi, Seiki
AU - Kawasaki, Hirofumi
PY - 1984/8
Y1 - 1984/8
N2 - Endogenous and 2-propanol-supported respiration of intact trophozoites of Entamoeba histolytica (stain HM-1:IMSS) were inhibited by bithionol, an effective chemotherapeutic agent for some trematode and cestode infections in humans. Dichlorophene and hexachlorophene also inhibited 2-propanol-supported respiration of the parasite. In contrast, ethanol formation by E. histolytica extract in the presence of N2 was scarcely inhibited by bithionol. The compound also inhibited in vitro growth of axenic (HM-1 strain) and polyxenic (strain HJ-1:KEIO) amoebae in culture. It took less than 24 hr to kill and disrupt virtually all amoebae of either strain with 0.28 mM bithionol. Omission of bovine serum from BI-S-33 medium resulted in considerably less disruption of HM-1 strain amoebae by the compound. However, organisms that looked undisrupted were stained with trypan blue. Moreover, the number of amoebae incubated for 10 min in the serum-free BI-S-33 medium containing 0.14 mM bithionol did not increase, even after incubation for 24 hr following replacement of the experimental culture fluid with fresh complete BI-S-33 medium free of the compound. These findings suggest that, although serum appears to diminish the antiamoebic action, some halogenated bisphenols (in particular bithionol) may be useful for treatment of amoebiasis.
AB - Endogenous and 2-propanol-supported respiration of intact trophozoites of Entamoeba histolytica (stain HM-1:IMSS) were inhibited by bithionol, an effective chemotherapeutic agent for some trematode and cestode infections in humans. Dichlorophene and hexachlorophene also inhibited 2-propanol-supported respiration of the parasite. In contrast, ethanol formation by E. histolytica extract in the presence of N2 was scarcely inhibited by bithionol. The compound also inhibited in vitro growth of axenic (HM-1 strain) and polyxenic (strain HJ-1:KEIO) amoebae in culture. It took less than 24 hr to kill and disrupt virtually all amoebae of either strain with 0.28 mM bithionol. Omission of bovine serum from BI-S-33 medium resulted in considerably less disruption of HM-1 strain amoebae by the compound. However, organisms that looked undisrupted were stained with trypan blue. Moreover, the number of amoebae incubated for 10 min in the serum-free BI-S-33 medium containing 0.14 mM bithionol did not increase, even after incubation for 24 hr following replacement of the experimental culture fluid with fresh complete BI-S-33 medium free of the compound. These findings suggest that, although serum appears to diminish the antiamoebic action, some halogenated bisphenols (in particular bithionol) may be useful for treatment of amoebiasis.
KW - Bithionol
KW - Chemotherapy
KW - Dichlorophene
KW - Entamoeba histolytica
KW - Hexachlorophene
KW - Protozoa
KW - Respiration
KW - parasitic
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U2 - 10.1016/0014-4894(84)90015-8
DO - 10.1016/0014-4894(84)90015-8
M3 - Article
C2 - 6086381
AN - SCOPUS:0021268334
VL - 58
SP - 1
EP - 7
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 1
ER -