Enzymatic inactivation of major circulating forms of atrial and brain natriuretic peptides

Junko Ozaki, Hiromasa Shimizu, Yukiya Hashimoto, Hiroshi Itoh, Kazuwa Nakao, Ken Ichi Inui

研究成果: Article査読

22 被引用数 (Scopus)

抄録

We compared the enzymatic inactivation of major circulating forms of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP). Both ANP and BNP induced a significant increase in cyclic GMP (cGMP) formation in cultured epithelial cell line derived from porcine kidney, LLC-PK1. The cGMP formation stimulated by ANP in LLC-PK1 cells was significantly decreased by pre-treatment of the peptide with rat renal brush-border membranes, and the inactivation of ANP was inhibited by neutral endopeptidase inhibitors, phosphoramidon and S-thiorphan. BNP exhibited greater resistance to enzymatic inactivation than did ANP. In addition, phosphoramidon potentiated the natriuresis with a low dose (7.5 pmol min-1 kg-1) of ANP but not of BNP in rats. These results suggest that enzymatic degradation of natriuretic peptides is highly dependent on peptide structure, and that the affinity of BNP to neutral endopeptidase is less than that of ANP. Copyright (C) 1999 Elsevier Science B.V.

本文言語English
ページ(範囲)307-312
ページ数6
ジャーナルEuropean journal of pharmacology
370
3
DOI
出版ステータスPublished - 1999 4月 16
外部発表はい

ASJC Scopus subject areas

  • 薬理学

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