Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone

Yuki Yoshimatsu, Rei Noguchi, Ryuto Tsuchiya, Takuya Ono, Yooksil Sin, Sei Akane, Jun Sugaya, Tomoaki Mori, Suguru Fukushima, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Giant cell tumor of bone (GCTB) is a locally aggressive and rarely metastasizing tumor. GCTB is characterized by the presence of unique giant cells and a recurrent mutation in the histone tail of the histone variant H3.3, which is encoded by H3F3A on chromosome 1. GCTB accounts for ~ 5% of primary bone tumors. Although GCTB exhibits an indolent course, it has the potential to develop aggressive behaviors associated with local recurrence and distant metastasis. Currently, complete surgical resection is the only curative treatment, and novel therapeutic strategies are required. Patient-derived cancer cell lines are critical tools for basic and pre-clinical research. However, only a few GCTB cell lines have been reported, and none of them are available from public cell banks. Therefore, we aimed to establish novel GCTB cell lines in the present study. Using curetted tumor tissues of GCTB, we established two cell lines and named them NCC-GCTB2-C1 and NCC-GCTB3-C1. These cells harbored a typical mutation in histones and exhibited slow but constant growth, formed spheroids, and had invasive capabilities. We demonstrated the utility of these cell lines for high-throughput drug screening using 214 anticancer agents. We concluded that NCC-GCTB2-C1 and NCC-GCTB3-C1 cell lines were useful for the in vitro study of GCTB.

本文言語English
ページ(範囲)1899-1910
ページ数12
ジャーナルHuman Cell
34
6
DOI
出版ステータスPublished - 2021 11月
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学
  • 癌研究

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