Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito, Toshihide Muramatsu, Hidetsugu Saito

研究成果: Article査読

3 被引用数 (Scopus)

抄録

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

本文言語English
論文番号100009
ジャーナルSTAR Protocols
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1
DOI
出版ステータスPublished - 2020 6月 19

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)

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