Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi, Tomoko Shofuda, Yohei Bamba, Atsuyo Yamamoto, Daisuke Kanematsu, Yukako Handa, Keisuke Okita, Masaya Nakamura, Shinya Yamanaka, Hideyuki Okano, Yonehiro Kanemura

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

本文言語English
論文番号7235757
ジャーナルStem Cells International
2016
DOI
出版ステータスPublished - 2016

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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