Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming

Mayutaka Nakajima, Sho Yoshimatsu, Tsukika Sato, Mari Nakamura, Junko Okahara, Erika Sasaki, Seiji Shiozawa, Hideyuki Okano

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The common marmoset (marmoset; Callithrix jacchus) shows anatomical and physiological features that are in common with humans. Establishing induced pluripotent stem cells (iPSCs) from marmosets holds promise for enhancing the utility of the animal model for biomedical and preclinical studies. However, in spite of the presence of some previous reports on marmoset iPSCs, the reprogramming technology in marmosets is still under development. In particular, the efficacy of RNA-based reprogramming has not been thoroughly investigated. In this study, we attempted RNA-based reprogramming for deriving iPSCs from marmoset fibroblasts. Although we failed to derive iPSC colonies from marmoset fibroblasts by using a conventional RNA-based reprogramming method previously validated in human fibroblasts, we succeeded in deriving colony-forming cells with a modified induction medium supplemented with a novel set of small molecules. Importantly, following one-week culture of the colony-forming cells in conventional embryonic stem cell (ESC) medium, we obtained iPSCs which express endogenous pluripotent markers and show a differentiation potential into all three germ layers. Taken together, our results indicate that RNA-based reprogramming, which is valuable for deriving transgene-free iPSCs, is applicable to marmosets.

本文言語English
ページ(範囲)593-599
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
515
4
DOI
出版ステータスPublished - 2019 8 6

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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