Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins

Takeshi Harayama, Hideo Shindou, Yoshihiro Kita, Eiji Otsubo, Kazushige Ikeda, Shoichi Chida, Timothy E. Weaver, Takao Shimizu

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The surfactant proteins (SPs), SP-B and SP-C, are important components of pulmonary surfactant involved in the reduction of alveolar surface tension. Quantification of SP-B and SP-C in surfactant drugs is informative for their quality control and the evaluation of their biological activity. Western blot analysis enabled the quantification of SP-B, but not SP-C, in surfactant drugs. Here, we report a new procedure involving chemical treatments and LC-MS to analyze SP-C peptides. The procedure enabled qualitative analysis of SP-C from different species with discrimination of the palmitoylation status and the artificial modifications that occur during handling and/or storage. In addition, the method can be used to estimate the total amount of SP-C in pulmonary surfactant drugs. The strategy described here might serve as a prototype to establish analytical methods for peptides that are extremely hydrophobic and behave like lipids. The new method provides an easy measurement of SP-C from various biological samples, which will help the characterization of various experimental animal models and the quality control of surfactant drugs, as well as diagnostics of human samples.

本文言語English
ページ(範囲)1370-1379
ページ数10
ジャーナルJournal of lipid research
56
7
DOI
出版ステータスPublished - 2015 7 1

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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