Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells

Kouichi Yanagi, Toru Komatsu, Shusuke Ogihara, Takayoshi Okabe, Hirotatsu Kojima, Tetsuo Nagano, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

研究成果: Article査読

抄録

In this study, we present a live-cell-based fluorometric coupled assay system to identify the compounds that can regulate the targeted metabolic pathways in live cells. The assay is established through targeting specific metabolic pathways and using “input” and “output” metabolite pairs. The changes in the extracellular output that are generated and released into the extracellular media from the input are assessed as the activity of the pathway. The screening for the glycolytic pathway and amino acid metabolism reveals the activities of the present drugs, 6-BIO and regorafenib, that regulate the metabolic fate of tumor cells.

本文言語English
論文番号109311
ジャーナルCell Reports
36
1
DOI
出版ステータスPublished - 2021 7月 6
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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