Evaluation of drug toxicity profiles based on the phenotypes of ascidian Ciona intestinalis

Yuji Mizotani, Shun Itoh, Kohji Hotta, Etsu Tashiro, Kotaro Oka, Masaya Imoto

研究成果: Article査読

2 被引用数 (Scopus)

抄録

In vivo toxicity evaluation using model organisms is an important step for the development of new drugs. Here, we report that Ciona intestinalis, a chordate invertebrate, is beneficial to drug toxicity evaluation for the following reasons: rapid embryonic and larval development, resemblance to vertebrates, ease of management, low cost, transparent body, and low risk of ethical issues. The dynamic phenotypic change of Ciona larvae during metamorphosis prompted us to examine the effect of cytotoxic drugs on its development by quantifying six toxicity endpoints: degenerated tail size, ampulla length, rotation of body axis, stomach size, heart rate, and body size. As a result, mitochondrial respiratory inhibitors, tubulin polymerization/depolymerization inhibitors, or DNA/RNA synthesis inhibitors showed distinct toxicity profiles against these six endpoints, but drugs with the same targets showed a similar toxicity profile in Ciona. Our results suggest Ciona is an effective animal model for profiling drug toxicity and exploring the mechanisms of drugs with unknown targets.

本文言語English
ページ(範囲)656-660
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
463
4
DOI
出版ステータスPublished - 2015 7月 13

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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