Evaluation of electrical propagation delay with cardiomyocytes by photosensitization reaction in vitro

Marika Doi, Emiyu Ogawa, Tsunenori Arai

研究成果: Conference contribution


In order to study cardiomyocyte electrical conduction damage by a photosensitization reaction (PR) mostly comes from outside of the cells in a few minutes after the PR, we studied propagation delay of contact action potential with cardiomyocyte by the PR. To determine appropriate PR condition for tachyarrhythmia ablation, a precise electrophysiological experiment in vitro has been preferable. We measured the contact action potential using a microelectrode array system of which information may be correct than conventional Ca2+ measurement. We investigated the propagation delays of an evoked potential to evaluate the electrical conduction damage by the PR. Rat cardiomyocytes were cultivated for 5-7 days on a dish with which 64 electrodes were patterned, in an incubator controlled to 37°C, 5% CO2. The following conditions were used for the PR: 40 μg/ml talapordfin sodium and 290 mW/cm2, 40-78 J/cm2 for an irradiation. A 2D map was obtained to visualize the propagation delays of the evoked potential. The propagation speed, which was calculated based on the measured propagation delays, was decreased by about 30-50% on average of all electrodes after the PR. Therefore, we think 2D propagation delays measurement of the evoked potential with contact action potential measuring system might be available to evaluate the acute electrical conduction damage of cardiomyocyte by the PR.

ホスト出版物のタイトルOptical Interactions with Tissue and Cells XXVIII
編集者E. Duco Jansen, Hope Thomas Beier
出版ステータスPublished - 2017
イベントOptical Interactions with Tissue and Cells XXVIII - San Francisco, United States
継続期間: 2017 1月 302017 1月 31


名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE


OtherOptical Interactions with Tissue and Cells XXVIII
国/地域United States
CitySan Francisco

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング


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