Evaluation of IS1245 LAMP in Mycobacterium avium and the influence of host-related genetic diversity on its application

Mwangala Lonah Akapelwa, Thoko Flav Kapalamula, Yuki Ouchi-Aizu, Bernard Mudenda Hang'ombe, Yukiko Nishiuchi, Stephen V. Gordon, Eddie Samuneti Solo, Aki Tamaru, Tomoyasu Nishimura, Naoki Hasegawa, Kozo Morimoto, Yukari Fukushima, Yasuhiko Suzuki, Chie Nakajima

研究成果: Article査読

抄録

Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts.

本文言語English
論文番号115494
ジャーナルDiagnostic Microbiology and Infectious Disease
101
4
DOI
出版ステータスPublished - 2021 12

ASJC Scopus subject areas

  • 微生物学(医療)
  • 感染症

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