Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase

Hideyuki Takahashi, Mitsunori Hayashi, Fumiyuki Goto, Shigeru Sato, Tomoyoshi Soga, Takaaki Nishioka, Masaru Tomita, Maki Kawai-Yamada, Hirofumi Uchimiya

研究成果: Article

56 引用 (Scopus)

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Background and Aims: Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars. Methods: Using CE/MS, we analysed several metabolites of glycolysis, the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. In addition, the concentrations of sugars and ion were quantified. Key Results: In YK1 (DFR)-overexpressing plants, the concentrations of cis-aconitate, isocitrate and 2-oxoglutarate were higher in leaves, whereas those of fructose-1,6- bisphosphate and glyceraldehyde-3-phosphate were lower in roots. In seeds, the amounts of free amino acids and metals were altered, whereas sugars in seeds were kept constant. In YK1 calli, an approx. 3-fold increase in glutathione was observed, whereas the activities of glutathione peroxidase and glutathione reductase were concomitantly increased. Conclusions: The overexpression of YK1 (DFR) was associated with slight changes in the amounts of several metabolites analysed in whole plants, whilst glutathione derivatives were substantially increased in suspension-cultured cells.

元の言語English
ページ(範囲)819-825
ページ数7
ジャーナルAnnals of Botany
98
発行部数4
DOI
出版物ステータスPublished - 2006 10

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genetically modified organisms
metabolites
rice
capillary electrophoresis
mass spectrometry
glutathione
glyceraldehyde 3-phosphate
ions
sugars
amino sugars
pentoses
tricarboxylic acid cycle
glycolysis
biotic stress
glutathione-disulfide reductase
seeds
glutathione peroxidase
stress tolerance
free amino acids
cultured cells

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

これを引用

Takahashi, H., Hayashi, M., Goto, F., Sato, S., Soga, T., Nishioka, T., ... Uchimiya, H. (2006). Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. Annals of Botany, 98(4), 819-825. https://doi.org/10.1093/aob/mcl162

Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. / Takahashi, Hideyuki; Hayashi, Mitsunori; Goto, Fumiyuki; Sato, Shigeru; Soga, Tomoyoshi; Nishioka, Takaaki; Tomita, Masaru; Kawai-Yamada, Maki; Uchimiya, Hirofumi.

:: Annals of Botany, 巻 98, 番号 4, 10.2006, p. 819-825.

研究成果: Article

Takahashi, H, Hayashi, M, Goto, F, Sato, S, Soga, T, Nishioka, T, Tomita, M, Kawai-Yamada, M & Uchimiya, H 2006, 'Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase', Annals of Botany, 巻. 98, 番号 4, pp. 819-825. https://doi.org/10.1093/aob/mcl162
Takahashi, Hideyuki ; Hayashi, Mitsunori ; Goto, Fumiyuki ; Sato, Shigeru ; Soga, Tomoyoshi ; Nishioka, Takaaki ; Tomita, Masaru ; Kawai-Yamada, Maki ; Uchimiya, Hirofumi. / Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. :: Annals of Botany. 2006 ; 巻 98, 番号 4. pp. 819-825.
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abstract = "Background and Aims: Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars. Methods: Using CE/MS, we analysed several metabolites of glycolysis, the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. In addition, the concentrations of sugars and ion were quantified. Key Results: In YK1 (DFR)-overexpressing plants, the concentrations of cis-aconitate, isocitrate and 2-oxoglutarate were higher in leaves, whereas those of fructose-1,6- bisphosphate and glyceraldehyde-3-phosphate were lower in roots. In seeds, the amounts of free amino acids and metals were altered, whereas sugars in seeds were kept constant. In YK1 calli, an approx. 3-fold increase in glutathione was observed, whereas the activities of glutathione peroxidase and glutathione reductase were concomitantly increased. Conclusions: The overexpression of YK1 (DFR) was associated with slight changes in the amounts of several metabolites analysed in whole plants, whilst glutathione derivatives were substantially increased in suspension-cultured cells.",
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AU - Hayashi, Mitsunori

AU - Goto, Fumiyuki

AU - Sato, Shigeru

AU - Soga, Tomoyoshi

AU - Nishioka, Takaaki

AU - Tomita, Masaru

AU - Kawai-Yamada, Maki

AU - Uchimiya, Hirofumi

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AB - Background and Aims: Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars. Methods: Using CE/MS, we analysed several metabolites of glycolysis, the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. In addition, the concentrations of sugars and ion were quantified. Key Results: In YK1 (DFR)-overexpressing plants, the concentrations of cis-aconitate, isocitrate and 2-oxoglutarate were higher in leaves, whereas those of fructose-1,6- bisphosphate and glyceraldehyde-3-phosphate were lower in roots. In seeds, the amounts of free amino acids and metals were altered, whereas sugars in seeds were kept constant. In YK1 calli, an approx. 3-fold increase in glutathione was observed, whereas the activities of glutathione peroxidase and glutathione reductase were concomitantly increased. Conclusions: The overexpression of YK1 (DFR) was associated with slight changes in the amounts of several metabolites analysed in whole plants, whilst glutathione derivatives were substantially increased in suspension-cultured cells.

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