Functionality Expression Institution that was newly enforced in April 2015 enabled the expression of functionality of not only the Food for Specified Health Uses, the Food with Nutrient Function Claims, but also a lot of foods including supplements. In order to obtain approval and authorization, it is necessary to release data that substantiate the efficacy, safety, and quality. Thus, the quantitative analysis of functional components in complex matrices must be carried out. For consumers, reliable data are useful for selecting favorite supplements that fit their needs. We have already reported a rapid quantitative analysis of multi-components in complex type supplements using ultra-high speed liquid chromatography. Although there are several activity evaluation methods for supplements in foods, few reports have shown the influences of co-existence of multi-components toward the functionality of the products. Therefore, we investigated and evaluated total antioxidant effect of complex-type supplements using various measurements of the antioxidative activity accompanied by close monitoring of the amounts of contained active components.
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