抄録
Small-angle X-ray scattering and nuclear magnetic resonance were used to investigate the structural change of calcium-bound calmodulin (Ca2+/CaM) in solution upon binding to its antagonist, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7). The radius of gyration was 17.4±0.3 A for Ca2+/CaM-W-7 with a molar ratio of 1:5 and 20.3±0.7 A for Ca2+/CaM. Comparison of the radius of gyration and the pair distance distribution function of the Ca2+/CaM-W-7 complex with those of other complexes indicates that binding of two W-7 molecules induces a globular shape for Ca2+/CaM, probably caused by an inter-domain compaction. The results suggest a tendency for Ca2+/CaM to form a globular structure in solution, which is inducible by a small compound like W-7. Copyright (C) 1999 Federation of European Biochemical Societies.
本文言語 | English |
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ページ(範囲) | 173-177 |
ページ数 | 5 |
ジャーナル | FEBS Letters |
巻 | 442 |
号 | 2-3 |
DOI | |
出版ステータス | Published - 1999 1 15 |
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology