Evolution of sequence recognition by restriction-modification enzymes: Selective pressure for specificity decrease

Akito Chinen, Yasuhiro Naito, Naofumi Handa, Ichizo Kobayashi

研究成果: Article

19 引用 (Scopus)

抄録

Several type II restriction-modification (RM) gene complexes kill host bacterial cells that have lost them, through attack on the chromosomal recognition sites of these cells. Two RM gene complexes recognizing the same sequence cannot simultaneously enjoy such stabilization through postsegregational host killing, because one will defend chromosomal sites from attack by the other. In the present work, we analyzed intrahost competition between two RM gene complexes when the recognition sequence of one was included in that of the other. When the EcoRII gene complex, recognizing 5'-CCWGG (W = A, T), is lost from the host, the SsoII gene complex, which recognizes 5'-CCNGG (N = A, T, G, C), will prevent host death by protecting CCWGG sites on the chromosome. However, when the SsoII (CCNGG) gene complex is lost, the EcoRII (CCWGG) gene complex will be unable to prevent host death through attack by SsoII on 5'-CCSGG (S = C, G) sites. These predictions were verified in our experiments, in which we analyzed plasmid maintenance, cell growth, cell shape, and chromosomal DNA. Our results demonstrate the presence of selective pressure for decrease in the specificity of recognition sequence of RM systems in the absence of invading DNA.

元の言語English
ページ(範囲)1610-1619
ページ数10
ジャーナルMolecular Biology and Evolution
17
発行部数11
出版物ステータスPublished - 2000

Fingerprint

Genes
enzyme
gene
Enzymes
enzymes
genes
DNA Restriction-Modification Enzymes
death
DNA
Cell Shape
restriction endonucleases
Cell growth
cells
Chromosomes
plasmid
chromosome
cell growth
plasmids
stabilization
Plasmids

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

これを引用

Evolution of sequence recognition by restriction-modification enzymes : Selective pressure for specificity decrease. / Chinen, Akito; Naito, Yasuhiro; Handa, Naofumi; Kobayashi, Ichizo.

:: Molecular Biology and Evolution, 巻 17, 番号 11, 2000, p. 1610-1619.

研究成果: Article

@article{ce3808ffcb754502941015b034b9cb3b,
title = "Evolution of sequence recognition by restriction-modification enzymes: Selective pressure for specificity decrease",
abstract = "Several type II restriction-modification (RM) gene complexes kill host bacterial cells that have lost them, through attack on the chromosomal recognition sites of these cells. Two RM gene complexes recognizing the same sequence cannot simultaneously enjoy such stabilization through postsegregational host killing, because one will defend chromosomal sites from attack by the other. In the present work, we analyzed intrahost competition between two RM gene complexes when the recognition sequence of one was included in that of the other. When the EcoRII gene complex, recognizing 5'-CCWGG (W = A, T), is lost from the host, the SsoII gene complex, which recognizes 5'-CCNGG (N = A, T, G, C), will prevent host death by protecting CCWGG sites on the chromosome. However, when the SsoII (CCNGG) gene complex is lost, the EcoRII (CCWGG) gene complex will be unable to prevent host death through attack by SsoII on 5'-CCSGG (S = C, G) sites. These predictions were verified in our experiments, in which we analyzed plasmid maintenance, cell growth, cell shape, and chromosomal DNA. Our results demonstrate the presence of selective pressure for decrease in the specificity of recognition sequence of RM systems in the absence of invading DNA.",
keywords = "Molecular evolution, Restriction and modification, Sequence recognition",
author = "Akito Chinen and Yasuhiro Naito and Naofumi Handa and Ichizo Kobayashi",
year = "2000",
language = "English",
volume = "17",
pages = "1610--1619",
journal = "Molecular Biology and Evolution",
issn = "0737-4038",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Evolution of sequence recognition by restriction-modification enzymes

T2 - Selective pressure for specificity decrease

AU - Chinen, Akito

AU - Naito, Yasuhiro

AU - Handa, Naofumi

AU - Kobayashi, Ichizo

PY - 2000

Y1 - 2000

N2 - Several type II restriction-modification (RM) gene complexes kill host bacterial cells that have lost them, through attack on the chromosomal recognition sites of these cells. Two RM gene complexes recognizing the same sequence cannot simultaneously enjoy such stabilization through postsegregational host killing, because one will defend chromosomal sites from attack by the other. In the present work, we analyzed intrahost competition between two RM gene complexes when the recognition sequence of one was included in that of the other. When the EcoRII gene complex, recognizing 5'-CCWGG (W = A, T), is lost from the host, the SsoII gene complex, which recognizes 5'-CCNGG (N = A, T, G, C), will prevent host death by protecting CCWGG sites on the chromosome. However, when the SsoII (CCNGG) gene complex is lost, the EcoRII (CCWGG) gene complex will be unable to prevent host death through attack by SsoII on 5'-CCSGG (S = C, G) sites. These predictions were verified in our experiments, in which we analyzed plasmid maintenance, cell growth, cell shape, and chromosomal DNA. Our results demonstrate the presence of selective pressure for decrease in the specificity of recognition sequence of RM systems in the absence of invading DNA.

AB - Several type II restriction-modification (RM) gene complexes kill host bacterial cells that have lost them, through attack on the chromosomal recognition sites of these cells. Two RM gene complexes recognizing the same sequence cannot simultaneously enjoy such stabilization through postsegregational host killing, because one will defend chromosomal sites from attack by the other. In the present work, we analyzed intrahost competition between two RM gene complexes when the recognition sequence of one was included in that of the other. When the EcoRII gene complex, recognizing 5'-CCWGG (W = A, T), is lost from the host, the SsoII gene complex, which recognizes 5'-CCNGG (N = A, T, G, C), will prevent host death by protecting CCWGG sites on the chromosome. However, when the SsoII (CCNGG) gene complex is lost, the EcoRII (CCWGG) gene complex will be unable to prevent host death through attack by SsoII on 5'-CCSGG (S = C, G) sites. These predictions were verified in our experiments, in which we analyzed plasmid maintenance, cell growth, cell shape, and chromosomal DNA. Our results demonstrate the presence of selective pressure for decrease in the specificity of recognition sequence of RM systems in the absence of invading DNA.

KW - Molecular evolution

KW - Restriction and modification

KW - Sequence recognition

UR - http://www.scopus.com/inward/record.url?scp=0033745660&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033745660&partnerID=8YFLogxK

M3 - Article

C2 - 11070049

AN - SCOPUS:0033745660

VL - 17

SP - 1610

EP - 1619

JO - Molecular Biology and Evolution

JF - Molecular Biology and Evolution

SN - 0737-4038

IS - 11

ER -