Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin

Yoshitaka Miyagawa, Nobutaka Kiyokawa, Nakaba Ochiai, Ken Ichi Imadome, Yasuomi Horiuchi, Keiko Onda, Misako Yajima, Hiroyuki Nakamura, Yohko U. Katagiri, Hajime Okita, Tomohiro Morio, Norio Shimizu, Junichiro Fujimoto, Shigeyoshi Fujiwara

研究成果: Article

11 引用 (Scopus)

抄録

Summary With an increase in the importance of umbilical cord blood (CB) as an alternative source of haematopoietic progenitors for allogenic transplantation, donor lymphocyte infusion (DLI) with donor CB-derived activated CD4+ T cells in the unrelated CB transplantation setting is expected to be of increased usefulness as a direct approach for improving post-transplant immune function. To clarify the characteristics of activated CD4+ T cells derived from CB, we investigated their mRNA expression profiles and compared them with those of peripheral blood (PB)-derived activated CD4+ T cells. Based on the results of a DNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), a relatively high level of forkhead box protein 3 (Foxp3) gene expression and a relatively low level of interleukin (IL)-17 gene expression were revealed to be significant features of the gene expression profile of CB-derived activated CD4+ T cells. Flow cytometric analysis further revealed protein expression of Foxp3 in a portion of CB-derived activated CD4+ T cells. The low level of retinoic acid receptor-related orphan receptor γ isoform t (RORγt) gene expression in CB-derived activated CD4+ T cells was speculated to be responsible for the low level of IL-17 gene expression. Our data indicate a difference in gene expression between CD4 + T cells from CB and those from PB. The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4+ T cells may be a more appropriate source for DLI.

元の言語English
ページ(範囲)405-419
ページ数15
ジャーナルImmunology
128
発行部数3
DOI
出版物ステータスPublished - 2009 11
外部発表Yes

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Fetal Blood
Cytokines
T-Lymphocytes
Genes
Forkhead Transcription Factors
Gene Expression
Interleukin-17
Lymphocytes
Retinoic Acid Receptors
Homologous Transplantation
Regulatory T-Lymphocytes
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome
Real-Time Polymerase Chain Reaction
Protein Isoforms
Transplantation
Transplants
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

これを引用

Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin. / Miyagawa, Yoshitaka; Kiyokawa, Nobutaka; Ochiai, Nakaba; Imadome, Ken Ichi; Horiuchi, Yasuomi; Onda, Keiko; Yajima, Misako; Nakamura, Hiroyuki; Katagiri, Yohko U.; Okita, Hajime; Morio, Tomohiro; Shimizu, Norio; Fujimoto, Junichiro; Fujiwara, Shigeyoshi.

:: Immunology, 巻 128, 番号 3, 11.2009, p. 405-419.

研究成果: Article

Miyagawa, Y, Kiyokawa, N, Ochiai, N, Imadome, KI, Horiuchi, Y, Onda, K, Yajima, M, Nakamura, H, Katagiri, YU, Okita, H, Morio, T, Shimizu, N, Fujimoto, J & Fujiwara, S 2009, 'Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin', Immunology, 巻. 128, 番号 3, pp. 405-419. https://doi.org/10.1111/j.1365-2567.2009.03122.x
Miyagawa, Yoshitaka ; Kiyokawa, Nobutaka ; Ochiai, Nakaba ; Imadome, Ken Ichi ; Horiuchi, Yasuomi ; Onda, Keiko ; Yajima, Misako ; Nakamura, Hiroyuki ; Katagiri, Yohko U. ; Okita, Hajime ; Morio, Tomohiro ; Shimizu, Norio ; Fujimoto, Junichiro ; Fujiwara, Shigeyoshi. / Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin. :: Immunology. 2009 ; 巻 128, 番号 3. pp. 405-419.
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abstract = "Summary With an increase in the importance of umbilical cord blood (CB) as an alternative source of haematopoietic progenitors for allogenic transplantation, donor lymphocyte infusion (DLI) with donor CB-derived activated CD4+ T cells in the unrelated CB transplantation setting is expected to be of increased usefulness as a direct approach for improving post-transplant immune function. To clarify the characteristics of activated CD4+ T cells derived from CB, we investigated their mRNA expression profiles and compared them with those of peripheral blood (PB)-derived activated CD4+ T cells. Based on the results of a DNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), a relatively high level of forkhead box protein 3 (Foxp3) gene expression and a relatively low level of interleukin (IL)-17 gene expression were revealed to be significant features of the gene expression profile of CB-derived activated CD4+ T cells. Flow cytometric analysis further revealed protein expression of Foxp3 in a portion of CB-derived activated CD4+ T cells. The low level of retinoic acid receptor-related orphan receptor γ isoform t (RORγt) gene expression in CB-derived activated CD4+ T cells was speculated to be responsible for the low level of IL-17 gene expression. Our data indicate a difference in gene expression between CD4 + T cells from CB and those from PB. The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4+ T cells may be a more appropriate source for DLI.",
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T1 - Ex vivo expanded cord blood CD4 T lymphocytes exhibit a distinct expression profile of cytokine-related genes from those of peripheral blood origin

AU - Miyagawa, Yoshitaka

AU - Kiyokawa, Nobutaka

AU - Ochiai, Nakaba

AU - Imadome, Ken Ichi

AU - Horiuchi, Yasuomi

AU - Onda, Keiko

AU - Yajima, Misako

AU - Nakamura, Hiroyuki

AU - Katagiri, Yohko U.

AU - Okita, Hajime

AU - Morio, Tomohiro

AU - Shimizu, Norio

AU - Fujimoto, Junichiro

AU - Fujiwara, Shigeyoshi

PY - 2009/11

Y1 - 2009/11

N2 - Summary With an increase in the importance of umbilical cord blood (CB) as an alternative source of haematopoietic progenitors for allogenic transplantation, donor lymphocyte infusion (DLI) with donor CB-derived activated CD4+ T cells in the unrelated CB transplantation setting is expected to be of increased usefulness as a direct approach for improving post-transplant immune function. To clarify the characteristics of activated CD4+ T cells derived from CB, we investigated their mRNA expression profiles and compared them with those of peripheral blood (PB)-derived activated CD4+ T cells. Based on the results of a DNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), a relatively high level of forkhead box protein 3 (Foxp3) gene expression and a relatively low level of interleukin (IL)-17 gene expression were revealed to be significant features of the gene expression profile of CB-derived activated CD4+ T cells. Flow cytometric analysis further revealed protein expression of Foxp3 in a portion of CB-derived activated CD4+ T cells. The low level of retinoic acid receptor-related orphan receptor γ isoform t (RORγt) gene expression in CB-derived activated CD4+ T cells was speculated to be responsible for the low level of IL-17 gene expression. Our data indicate a difference in gene expression between CD4 + T cells from CB and those from PB. The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4+ T cells may be a more appropriate source for DLI.

AB - Summary With an increase in the importance of umbilical cord blood (CB) as an alternative source of haematopoietic progenitors for allogenic transplantation, donor lymphocyte infusion (DLI) with donor CB-derived activated CD4+ T cells in the unrelated CB transplantation setting is expected to be of increased usefulness as a direct approach for improving post-transplant immune function. To clarify the characteristics of activated CD4+ T cells derived from CB, we investigated their mRNA expression profiles and compared them with those of peripheral blood (PB)-derived activated CD4+ T cells. Based on the results of a DNA microarray analysis and quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR), a relatively high level of forkhead box protein 3 (Foxp3) gene expression and a relatively low level of interleukin (IL)-17 gene expression were revealed to be significant features of the gene expression profile of CB-derived activated CD4+ T cells. Flow cytometric analysis further revealed protein expression of Foxp3 in a portion of CB-derived activated CD4+ T cells. The low level of retinoic acid receptor-related orphan receptor γ isoform t (RORγt) gene expression in CB-derived activated CD4+ T cells was speculated to be responsible for the low level of IL-17 gene expression. Our data indicate a difference in gene expression between CD4 + T cells from CB and those from PB. The findings of Foxp3 expression, a characteristic of regulatory T cells, and a low level of IL-17 gene expression suggest that CB-derived CD4+ T cells may be a more appropriate source for DLI.

KW - CD4

KW - Cord blood

KW - Donor lymphocyte infusion

KW - Forkhead box protein 3

KW - Interleukin 17

KW - T cell

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