Excessive neutrophil elastase in bronchoalveolar lavage fluid in subclinical emphysema

Aya Yoshioka, Tomoko Betsuyaku, Masaharu Nishimura, Kenji Miyamoto, Takahito Kondo, Yoshikazu Kawakami

研究成果: Article

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In an attempt to further evaluate the role of neutrophil elastase (NE) in the development of emphysema, we examined the immunologic quantity of NE bound to α1-protease inhibitor (PI), the NE inhibitory activity, and the molecular pattern of α1-PI in unconcentrated bronchoalveolar lavage fluid (BALF) supernatant from 36 community-based older volunteers. They were classified into three groups: 10 current smokers with low attenuation areas (LAAs) on the lung computed tomography (CT) scans who were considered to have subclinical emphysema, 13 current smokers who had a comparable smoking history but no LAA, and 13 noncurrent smokers without LAA. The concentration of NE-α1-PI complex was significantly increased in the subjects with subclinical emphysema when compared not only with the noncurrent smokers (0.52 ± 0.10 versus 0.21 ± 0.03 SEM μg/mg albumin, p < 0.01) but also with the LAA(-) current smokers (0.52 ± 0.10 versus 0.23 ± 0.07 SEM μg/mg albumin, p < 0.01). NE inhibitory activity measured by a spectrophotometric method using methoxysuccinyl-alanyl-alanyl-prolyl-valyl-paranitroanilide did not show any significant difference between the two groups of current smokers. There was no difference in the pattern or density of native and proteolysed α1-PI bands between the three groups by Western blotting. We conclude that NE-α1-PI complex in BALF is a factor that may differentiate smokers who are potentially developing emphysema from those who are not.

元の言語English
ページ(範囲)2127-2132
ページ数6
ジャーナルAmerican journal of respiratory and critical care medicine
152
発行部数6 I
DOI
出版物ステータスPublished - 1995 12

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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