Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc

Daisuke Sakai, Yoshihiko Nakamura, Tomoko Nakai, Taishi Mishima, Shunichi Kato, Sibylle Grad, Mauro Alini, Makarand V. Risbud, Danny Chan, Kathryn S.E. Cheah, Ken Ichi Yamamura, Koichi Masuda, Hideyuki Okano, Kiyoshi Ando, Joji Mochida

研究成果: Article査読

252 被引用数 (Scopus)

抄録

Despite the high prevalence of intervertebral disc disease, little is known about changes in intervertebral disc cells and their regenerative potential with ageing and intervertebral disc degeneration. Here we identify populations of progenitor cells that are Tie2 positive (Tie2+) and disialoganglioside 2 positive (GD2+), in the nucleus pulposus from mice and humans. These cells form spheroid colonies that express type II collagen and aggrecan. They are clonally multipotent and differentiated into mesenchymal lineages and induced reorganization of nucleus pulposus tissue when transplanted into non-obese diabetic/severe combined immunodeficient mice. The frequency of Tie2+ cells in tissues from patients decreases markedly with age and degeneration of the intervertebral disc, suggesting exhaustion of their capacity for regeneration. However, progenitor cells (Tie2+ GD2+) can be induced from their precursor cells (Tie2+ GD2-) under simple culture conditions. Moreover, angiopoietin-1, a ligand of Tie2, is crucial for the survival of nucleus pulposus cells. Our results offer insights for regenerative therapy and a new diagnostic standard.

本文言語English
論文番号1264
ジャーナルNature communications
3
DOI
出版ステータスPublished - 2012

ASJC Scopus subject areas

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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