Exogenous insulin dose-dependently suppresses glucopenia-induced glucagon secretion from perfused rat pancreas

Katsuhiko Ito, Hiroshi Maruyama, Hiroshi Hirose, Koichi Kido, Kazunori Koyama, Kunizo Kataoka, Takao Saruta

研究成果: Article査読

24 被引用数 (Scopus)

抄録

To clarify the role of insulin in modulating the glucagon response to glucose concentration changes, we investigated the effects of exogenous insulin (10 mU/mL, 100 mU/mL, and 3.3 U/mL) on responses to high glucose (5.6 → 16.7 mmol/L), low glucose (5.6 → 1.4 mmol/L), and arginine (10 mmol/L) stimulation using the perfused rat pancreas. Although glucagon levels were slightly suppressed by all of the exogenous insulin concentrations tested for the initial few minutes at 5.6 mmol/L glucose, baseline levels were maintained thereafter. Glucagon responses to high or normal glucose concentrations were not altered, but glucopenia-induced glucagon secretion was significantly suppressed as compared with that of controls (0.77 ± 0.14 ng/min [10 mU/mL, n = 5], 0.55 ± 0.14 ng/min [100 mU/mL, n = 5], 0.27 ± 0.13 ng/min [3.3 U/mL, n = 5] v 1.38 ± 0.20 ng/min [controls, n = 9], P < .05, respectively). The first phase of the glucagon response to arginine was potentiated (2.03 ± 0.24 v 1.17 ± 0.22 ng/min, P < .05) by 10 mU/mL exogenous insulin. The second phase of the glucagon response to arginine was significantly suppressed in the presence of higher concentrations of exogenous insulin (1.16 ± 0.23 ng/min [100 mU/mL], 0.96 ± 0.08 ng/min [3.3 U/mL] v 1.57 ± 0.17 ng/min, P < .05, respectively). These results suggest that glucagon secretion is modified by the combined suppressive effects of glucose and insulin, although it is mainly glucose that mediates glucagon secretion in the physiological glucose range. Glucopenia- or arginine-induced glucagon secretion is suppressed by insulin.

本文言語English
ページ(範囲)358-362
ページ数5
ジャーナルMetabolism
44
3
DOI
出版ステータスPublished - 1995 3

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 内分泌学

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