Expanding the Repertoire of Optogenetically Targeted Cells with an Enhanced Gene Expression System

Kenji F. Tanaka, Ko Matsui, Takuya Sasaki, Hiromi Sano, Shouta Sugio, Kai Fan, René Hen, Junichi Nakai, Yuchio Yanagawa, Hidetoshi Hasuwa, Masaru Okabe, Karl Deisseroth, Kazuhiro Ikenaka, Akihiro Yamanaka

研究成果: Article査読

108 被引用数 (Scopus)

抄録

Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.

本文言語English
ページ(範囲)397-406
ページ数10
ジャーナルCell Reports
2
2
DOI
出版ステータスPublished - 2012 8 30

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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