Immunostaining with NJ-1 monoclonal antibody (MoAb) revealed that NJ-1 is expressed on megakaryocytes (MKs). NJ-1-positive and lineage-negative progenitor cells have a higher potency to proliferate and differentiate into MKs. MKs were divided into NJ-1+MKs and NJ-1−MKs. NJ-1+MKs are immature MKs because of their low potential to generate proplatelets. When cultured CD41-positive MK cells were analyzed with RT-PCR, we found that the expression of NJ-1 is down-regulated. NJ-1+MKs have a high adherent potential to endothelial cells comparing with NJ-1−MKs, and this binding ability could be inhibited by the NJ-1-Fc fusion protein. We hypothesize that NJ-1+MKs are immature MKs and the NJ-1 molecule is involved in MK adhesion to endothelial cells.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||Published - 2002 1月 1|
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