抄録
In contrast to transport across basolateral membranes, the mechanism governing transport of organic anions across the luminal membranes of proximal tubules has remained unclear. We recently found Tetracycline transporter-like protein (TETRAN), a human ortholog of yeast Tpo1p that can transport anionic Non-steroidal anti-inflammatory drugs (NSAIDs). In this study, we examine the expression and function of TETRAN. TETRAN mRNA is expressed in various human tissues, including kidney. When overexpressed in cultured cells, TETRAN was predominantly localized on cytoplasmic membranes. Immunohistochemical analysis of human and mouse kidney tissue showed that TETRAN was expressed at the luminal membranes of proximal tubules. Overexpression of TETRAN in cultured cells facilitated the uptake of organic anions such as indomethacin (a NSAID) and fluorescein. The results suggest that TETRAN is a novel human organic anion transporter, and that it serves as a transporter for some NSAIDs and various other organic anions at the final excretion step.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 325-330 |
| ページ数 | 6 |
| ジャーナル | Biochemical and Biophysical Research Communications |
| 巻 | 374 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | Published - 2008 9 19 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
これを引用
Expression and function of TETRAN, a new type of membrane transporter. / Ushijima, Hironori; Hiasa, Miki; Namba, Takushi; Hwang, Hyun Jung; Hoshino, Tatsuya; Mima, Shinji; Tsuchiya, Tomofusa; Moriyama, Yoshinori; Mizushima, Tohru.
：: Biochemical and Biophysical Research Communications, 巻 374, 番号 2, 19.09.2008, p. 325-330.研究成果: Article
}
TY - JOUR
T1 - Expression and function of TETRAN, a new type of membrane transporter
AU - Ushijima, Hironori
AU - Hiasa, Miki
AU - Namba, Takushi
AU - Hwang, Hyun Jung
AU - Hoshino, Tatsuya
AU - Mima, Shinji
AU - Tsuchiya, Tomofusa
AU - Moriyama, Yoshinori
AU - Mizushima, Tohru
PY - 2008/9/19
Y1 - 2008/9/19
N2 - In contrast to transport across basolateral membranes, the mechanism governing transport of organic anions across the luminal membranes of proximal tubules has remained unclear. We recently found Tetracycline transporter-like protein (TETRAN), a human ortholog of yeast Tpo1p that can transport anionic Non-steroidal anti-inflammatory drugs (NSAIDs). In this study, we examine the expression and function of TETRAN. TETRAN mRNA is expressed in various human tissues, including kidney. When overexpressed in cultured cells, TETRAN was predominantly localized on cytoplasmic membranes. Immunohistochemical analysis of human and mouse kidney tissue showed that TETRAN was expressed at the luminal membranes of proximal tubules. Overexpression of TETRAN in cultured cells facilitated the uptake of organic anions such as indomethacin (a NSAID) and fluorescein. The results suggest that TETRAN is a novel human organic anion transporter, and that it serves as a transporter for some NSAIDs and various other organic anions at the final excretion step.
AB - In contrast to transport across basolateral membranes, the mechanism governing transport of organic anions across the luminal membranes of proximal tubules has remained unclear. We recently found Tetracycline transporter-like protein (TETRAN), a human ortholog of yeast Tpo1p that can transport anionic Non-steroidal anti-inflammatory drugs (NSAIDs). In this study, we examine the expression and function of TETRAN. TETRAN mRNA is expressed in various human tissues, including kidney. When overexpressed in cultured cells, TETRAN was predominantly localized on cytoplasmic membranes. Immunohistochemical analysis of human and mouse kidney tissue showed that TETRAN was expressed at the luminal membranes of proximal tubules. Overexpression of TETRAN in cultured cells facilitated the uptake of organic anions such as indomethacin (a NSAID) and fluorescein. The results suggest that TETRAN is a novel human organic anion transporter, and that it serves as a transporter for some NSAIDs and various other organic anions at the final excretion step.
KW - Cytoplasmic membranes
KW - Final excretion step
KW - Indomethacin
KW - Kidney
KW - Luminal membranes of proximal tubules
KW - Membrane transporter
KW - NSAIDs
KW - Organic anions
KW - TETRAN
KW - Tpo1p
UR - http://www.scopus.com/inward/record.url?scp=48349100205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48349100205&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.07.034
DO - 10.1016/j.bbrc.2008.07.034
M3 - Article
C2 - 18638446
AN - SCOPUS:48349100205
VL - 374
SP - 325
EP - 330
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -