Expression and subcellular distribution of the active form of c-Src tyrosine kinase in differentiating human endometrial stromal cells

Yurie Yamamoto, Tetsuo Maruyama, Nozomi Sakai, Rei Sakurai, Aki Shimizu, Toshio Hamatani, Hirotaka Masuda, Hiroshi Uchida, Hisataka Sabe, Yasunori Yoshimura

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Decidual growth factors and locally produced cytokines are thought to activate specific phosphorylation signalling pathway(s), thereby eliciting a variety of decidual functions. We have previously reported the activation of c-Src tyrosine kinase during ovarian steroid-induced decidualization of cultured human endometrial stromal cells. As chicken c-Src is known to be activated upon dephosphorylation of tyrosine 527 (Y527, corresponding to Y530 in human), we here employed a monoclonal antibody, clone 28, directed against the active form of human c-Src whose Y530 is dephosphorylated, and investigated whether c-Src became dephosphorylated at Y530 and thereby activated during decidualization. We found that the active form of c-Src was up-regulated and demonstrated increased kinase activity during in-vitro decidualization. Immunohistochemistry revealed that decidual cells in early pregnancy decidua were intensely stained with clone 28 when compared with the stromal cells in the non-pregnant endometrium. Moreover, the active form of c-Src translocated from a perinuclear region to the cytoplasm upon decidualization. Thus, the Y530 dephosphorylation, kinase activation, and subcellular translocation of c-Src may be intracellular signalling events associated with decidualization in vivo as well as in vitro.

本文言語English
ページ(範囲)1117-1124
ページ数8
ジャーナルMolecular Human Reproduction
8
12
DOI
出版ステータスPublished - 2002 12 1

ASJC Scopus subject areas

  • 生殖医学
  • 胎生学
  • 分子生物学
  • 遺伝学
  • 産婦人科学
  • 発生生物学
  • 細胞生物学

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