TY - JOUR
T1 - Expression of AIRE gene in peripheral monocyte/dendritic cell lineage
AU - Kogawa, Kazuhiko
AU - Nagafuchi, Seiho
AU - Katsuta, Hitoshi
AU - Kudoh, Jun
AU - Tamiya, Sadafumi
AU - Sakai, Yumiko
AU - Shimizu, Nobuyoshi
AU - Harada, Mine
N1 - Funding Information:
The authors thank Ms Yukari Ikeda for her excellent technical assistance. A part of this study was conducted at the Kyushu University Station for Collaborative Research. This work was supported in part by a grant from Kyushu University Interdisciplinary Programs in Education and Projects in Research Development. The work at the Keio University School of Medicine was supported in part by a Grant in Aid for Scientific Research and a Fund for ‘Research for the Future’ Program from the Japan Society for the Promotion of Science (JSPS).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/3/1
Y1 - 2002/3/1
N2 - The responsible gene for autoimmune polyglandular syndrome type 1, known as autoimmune regulator (AIRE), was identified by positional cloning. The AIRE gene was reported to be expressed in the thymus medulla and lymph nodes. However, an expression of the AIRE gene in peripheral blood cells has not yet been reported. In the present study, we found that the AIRE gene was restrictively expressed in peripheral CD14-positive monocytes but not in CD4-positive T cells nor polymorphonuclear cells, as assessed by RT-PCR. Moreover, immunocytochemical study revealed the expression of the AIRE protein not only in CD14-positive monocytes but also in differentiated dendritic cells, cultured in RPMI1640 medium containing 800 U/ml GM-CSF, 1000 U/ml IL-4 and 100 U/ml TNF-α. Thus, it was concluded that the AIRE gene is restrictively expressed in the peripheral monocyte/dendritic cell lineage.
AB - The responsible gene for autoimmune polyglandular syndrome type 1, known as autoimmune regulator (AIRE), was identified by positional cloning. The AIRE gene was reported to be expressed in the thymus medulla and lymph nodes. However, an expression of the AIRE gene in peripheral blood cells has not yet been reported. In the present study, we found that the AIRE gene was restrictively expressed in peripheral CD14-positive monocytes but not in CD4-positive T cells nor polymorphonuclear cells, as assessed by RT-PCR. Moreover, immunocytochemical study revealed the expression of the AIRE protein not only in CD14-positive monocytes but also in differentiated dendritic cells, cultured in RPMI1640 medium containing 800 U/ml GM-CSF, 1000 U/ml IL-4 and 100 U/ml TNF-α. Thus, it was concluded that the AIRE gene is restrictively expressed in the peripheral monocyte/dendritic cell lineage.
KW - Autoimmune polyglandular syndrome type 1
KW - Autoimmune regulator gene
KW - Autoimmune-polyendocrinopathy-candidiasis ectodermal dystrophy
KW - Dendritic cells
KW - Monocytes
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U2 - 10.1016/S0165-2478(01)00314-5
DO - 10.1016/S0165-2478(01)00314-5
M3 - Article
C2 - 11803052
AN - SCOPUS:0036498116
SN - 0165-2478
VL - 80
SP - 195
EP - 198
JO - Immunology Letters
JF - Immunology Letters
IS - 3
ER -