Expression of APG-2 protein, a member of the heat shock protein 110 family, in developing rat brain

Michiyo Okui, Fumiaki Ito, Kiyokazu Ogita, Nobuyuki Kuramoto, Jun Kudoh, Nobuyoshi Shimizu, Toshinori Ide

研究成果: Article査読

9 被引用数 (Scopus)

抄録

APG-2 protein is a member of the heat shock protein 110 family, and it is thought to play an important role in the maintenance of neuronal functions under physiological and stress conditions. However, neither the tissue-distribution of APG-2 protein nor developmental change of its expression has been studied at the protein level. Therefore, we generated an antiserum against APG-2 protein and studied expression of this protein in rat brain and other tissues by use of the Western blot method. The results showed a high expression of APG-2 protein in various regions of the central nervous system (cerebral cortex, hippocampus, striatum, midbrain, hypothalamus, cerebellum, medulla pons, and spinal cord) throughout the entire postnatal stage. Similarly, a high level of APG-2 protein was detected in the whole brain of rat embryos and in adult rat tissues such as liver, lung, spleen, and kidney. In contrast, its expression in heart was high at postnatal days 1 and 3, but thereafter drastically decreased to a low level. Furthermore, APG-2 protein was detected in neuronal primary cultures prepared from rat cerebral cortex, and its level did not change notably during neuronal differentiation. These results show that APG-2 protein is constitutively expressed in various tissues and also in neuronal cells throughout the entire embryonic and postnatal period, suggesting that it might play an important role in these tissues under non-stress conditions. Copyright (C) 1999 Elsevier Science Ltd.

本文言語English
ページ(範囲)35-43
ページ数9
ジャーナルNeurochemistry International
36
1
DOI
出版ステータスPublished - 2000 1月
外部発表はい

ASJC Scopus subject areas

  • 細胞および分子神経科学
  • 細胞生物学

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