Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver

S. Moriya, S. Nagata, H. Yokoyama, S. Kato, Y. Horie, T. Ito, Y. Ebihara, H. Ishii

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Gamma-glutamyl transpeptidase (γ-GT) is the enzyme in the γ-glutamyl cycle which mediates the first step of glutathione degradation. Recently, it has been reported that diethyl maleate, a glutathione-depleting agent, enhanced hepatic γ-GT activity. In this study, we examined how γ-GT mRNA was expressed after the depletion of glutathione by using the RT-PCR method and in situ hybridization technique with digoxigenin-labeled oligonucleotide probe. We could detect hepatic γ-GT mRNA in the epithelial cell of bile duct and the cytoplasm of hepatocytes in the periportal area, even in control rats. Furthermore, we found that the expression of γ-GT mRNA was expanded to hepatocytes of a whole lobule 12 hr after depletion of glutathione by diethyl maleate, followed by the return to the pretreatment condition under which γ-GT mRNA appears to be controlled in correlation with hepatic glutathione levels. These results suggest that the depletion of glutathione may induce hepatic γ-GT activity through an increased synthesis of its mRNA.

本文言語English
ページ(範囲)107-111
ページ数5
ジャーナルAlcohol and Alcoholism
29
SUPPL. 1
出版ステータスPublished - 1994 12月 1

ASJC Scopus subject areas

  • 医学(その他)
  • 毒物学
  • 精神医学および精神衛生

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