Expression of heme oxygenase and its RNA in mouse liver after injection of heme and splenectomy

Shun ichi Kurata, Midori Matsumoto

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Heme is known to activate the HO (heme oxygenase) gene in cultured cells, but little is known about the effect of heme on the HO gene in intact organisms. The expressions of HO and its RNA in mouse liver were measured using mouse HO cDNA and HO antibody after injection of heme or splenectomy. The antibody was prepared against a β-galactosidase-HO hybrid protein made in Escherichia coli. The HO mRNA level increased to a maximum 15 h after heme injection. In contrast, expression of HO was maximal about 45 h after heme injection. Essentially the same results were obtained in mice after splenectomy. These results suggest that the HO gene in mouse liver was activated by the injection of heme and splenectomy.

本文言語English
ページ(範囲)255-258
ページ数4
ジャーナルBBA - Gene Structure and Expression
1132
3
DOI
出版ステータスPublished - 1992 10月 20
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

フィンガープリント

「Expression of heme oxygenase and its RNA in mouse liver after injection of heme and splenectomy」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル