We examined the expression of β 1,4-GT gene products in 11 gynecological cancer cell lines. A 4.7 kb mRNA and protein (54,000 Da and 57,000 Da) were detected by Northern blot and Western blot. Immunocytochemical staining revealed that β 1,4-GT was localized in the Golgi or ER of tumor cells. An intense β 1,4-GT mRNA signal was detected in ovarian and cervical cancer cells, whereas the level of β 1,4-GT mRNA was very low in uterine endometrial cancer cells. We also confirmed that expression of β 1,4-GT mRNA corresponded to expression of β 1,4-GT protein. These results suggest that expression of the β 1,4-GT gene products is higher in human cervical and ovarian cancer cells than in uterine endometrial cancer cells.
|ジャーナル||International journal of oncology|
|出版ステータス||Published - 1997|
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