Expression of LL-37 by Human Gastric Epithelial Cells as a Potential Host Defense Mechanism Against Helicobacter pylori

Koji Hase, Masamoto Murakami, Mitsutoshi Iimura, Sheri P. Cole, Yoshimune Horibe, Takaaki Ohtake, Marygorret Obonyo, Richard L. Gallo, Lars Eckmann, Martin F. Kagnoff

研究成果: Article査読

175 被引用数 (Scopus)

抄録

Background & Aims: LL-37/human cationic antimicrobial peptide 18 (hCAP18) is a human cathelicidin with broad-spectrum antimicrobial, lipopolysaccharide binding, and chemotactic activities. This study examined the role of LL-37/hCAP18 in gastric innate immune defense by characterizing its constitutive and regulated expression by human gastric mucosa and its bactericidal activity against the gastric pathogen Helicobacter pylori. Methods: LL-37/hCAP18 messenger RNA expression in normal and H. pylori-infected gastric mucosa and gastric epithelial cells was determined by in situ hybridization, real-time polymerase chain reaction, immunostaining, and immunoblot analysis. Bactericidal activity was measured by using a colony-forming unit assay. Results: LL-37/hCAP18 messenger RNA and protein were expressed in a distinct distribution by surface epithelial cells as well as chief and parietal cells in the fundic glands of normal gastric mucosa. LL-37/hCAP18 was significantly increased in the epithelium and gastric secretions of H. pylori-infected patients, but not in individuals with non-H. pylori-induced gastric inflammation. Infection of cultured gastric epithelial cells with a wild-type but not an isogenic ΔcagE mutant strain of H. pylori increased LL-37/hCAP18 expression, indicating that H. pylori-induced regulation of LL-37/hCAP18 production required an intact type IV secretion system. LL-37, the C-terminal peptide of LL-37/hCAP18, alone or in synergy with human β-defensin 1, was bactericidal for several H. pylori strains. Conclusions: These data indicate that H. pylori up-regulates production of LL-37/hCAP18 by gastric epithelium and suggest this cathelicidin contributes to determining the balance between host mucosal defense and H. pylori survival mechanisms that govern chronic infection with this gastric pathogen.

本文言語English
ページ(範囲)1613-1625
ページ数13
ジャーナルGastroenterology
125
6
DOI
出版ステータスPublished - 2003 12
外部発表はい

ASJC Scopus subject areas

  • 肝臓学
  • 消化器病学

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