Expression of membrane-bound transferrin-like protein p97 on the cell surface of chondrocytes

Takeshi Kawamoto, Haiou Pan, Weiqun Yan, Hideyuki Ishida, Emiko Usui, Ryo Oda, Kazuko Nakamasu, Mitsuhide Noshiro, Yoshie Kawashima-Ohya, Masashi Fujii, Hideaki Shintani, Yasunori Okada, Yukio Kato

研究成果: Article査読

28 被引用数 (Scopus)


A concanavalin-A-binding protein of 76 kDa was purified from the plasma membrane fraction of rabbit chondrocyte cultures. Amino acid sequencing of the N-terminal region and of tryptic peptides of the protein, in addition to sequencing of its cDNA revealed that this protein is highly similar to the tumour-associated antigen p97. Hence, it was concluded that this protein is the rabbit form of p97. Western blotting, Northern blotting and reverse- transcription PCR analyses indicated that rabbit p97 is expressed at high levels in cartilage and chondrocytes, but is barely detectable in the bone, liver, kidney, small intestine, eye, pancreas, heart, testis, skeletal muscle, spleen and fibroblasts. Immunocytochemical and immunohistochemical analyses demonstrated that p97 is expressed in the plasma membrane of chondrocytes. p97 transcript was detected in all zones of the cartilage but the level was relatively low in the hypertrophic zone. These findings suggest that p97 is involved in maintaining the cell surface characteristics of chondrocytes.

ジャーナルEuropean Journal of Biochemistry
出版ステータスPublished - 1998 9月 15

ASJC Scopus subject areas

  • 生化学


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