Expression of membrane-type matrix metalloproteinase 1 (MT1-MMP) in tumor cells enhances pulmonary metastasis in an experimental metastasis assay

Yoshio Tsunezuka, Hiroaki Kinoh, Takahisa Takino, Yoh Watanabe, Yasunori Okada, Akira Shinagawa, Hiroshi Sato, Motoharu Seiki

研究成果: Article査読

117 被引用数 (Scopus)

抄録

Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a member of the recently identified unique membrane-type subgroup in the matrix metalloproteinase (MMP) family. MT1-MMP has proteolytic activity against components in the extracellular matrix and activates progelatinase A (72-kDa type IV procollagenase/proMMP-2) on the cell surface. Because MT1-MMP is frequently expressed in a variety of tumors, we examined its contribution to their metastatic potential. The mouse lung carcinoma cell line Madison 109 was transiently transfected with a MT1-MMP expression plasmid and inoculated into the tail vein of BALB/c mouse. Fate of the transfected cells was monitored by the neo(r) gene in the plasmid using the quantitative PCR method. The survival rate of the parental cells in lung was 0.7% of the inoculated cells. It was increased by 3-fold with the MT1-MMP transfected cells and the number of the lung nodules increased accordingly. Immunostaining of the consecutive tissue sections revealed that lung nodules expressing MT1-MMP were positive for gelatinase A as well, whereas MT1-MMP- negative cells were not stained for gelatinase A at all. Thus, MT1-MMP- expressing cells acquire specific ability to bind exogenous progelatinase A.

本文言語English
ページ(範囲)5678-5683
ページ数6
ジャーナルCancer Research
56
24
出版ステータスPublished - 1996 12 15
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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