抄録
We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 3263-3266 |
| ページ数 | 4 |
| ジャーナル | Cancer Research |
| 巻 | 55 |
| 発行部数 | 15 |
| 出版物ステータス | Published - 1995 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
これを引用
Expression of membrane-type matrix metalloproteinase in human gastric carcinomas. / Nomura, H.; Sato, H.; Seiki, M.; Mat, M.; Okada, Y.
：: Cancer Research, 巻 55, 番号 15, 1995, p. 3263-3266.研究成果: Article
}
TY - JOUR
T1 - Expression of membrane-type matrix metalloproteinase in human gastric carcinomas
AU - Nomura, H.
AU - Sato, H.
AU - Seiki, M.
AU - Mat, M.
AU - Okada, Y.
PY - 1995
Y1 - 1995
N2 - We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.
AB - We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.
UR - http://www.scopus.com/inward/record.url?scp=0029103339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029103339&partnerID=8YFLogxK
M3 - Article
C2 - 7614460
AN - SCOPUS:0029103339
VL - 55
SP - 3263
EP - 3266
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 15
ER -