TY - JOUR
T1 - Expression of neural RNA-binding proteins in the postnatal CNS
T2 - Implications of their roles in neuronal and glial cell development
AU - Sakakibara, Shin Ichi
AU - Okano, Hideyuki
PY - 1997
Y1 - 1997
N2 - There is an increasing interest in the role of RNA-binding proteins during neural development. Mouse-Musashi-1 (m-Msi-1) is a mouse neural RNA- binding protein with sequence similarity to Drosophila musashi (d-msi), which is essential for neural development. m-Msi-1 is highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic CNS development. The present study characterized m-Msi-1-expressing cells in the postnatal and adult CNS. Postnatally, m-Msi-1 was expressed in proliferative neuronal precursors in the external granule cell layer of the cerebellum and in the anterior corner of the subventricular zone of the lateral ventricles. In gliogenesis, the persistent expression of m-Msi-1 was observed in cells of the astrocyte lineage ranging from proliferative glial precursors in the subventricular zone (SVZ), to differentiated astrocytes in the parenchyma. In addition, we showed that m-Mis-1 was still expressed in proliferating cells in the adult SVZ, which may contain neural precursor or stem cells. Another neural RNA-binding protein Hu (the mammalian homolog of Drosophila neuronal RNA-binding protein Elav) was present in postmitotic neurons throughout the development of the CNS, and its pattern of expression was compared with that of m-Msi-1. These observations imply that these two RNA-binding proteins may be involved in the development of neurons and glia by regulating gene expression at the post-transcriptional level.
AB - There is an increasing interest in the role of RNA-binding proteins during neural development. Mouse-Musashi-1 (m-Msi-1) is a mouse neural RNA- binding protein with sequence similarity to Drosophila musashi (d-msi), which is essential for neural development. m-Msi-1 is highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic CNS development. The present study characterized m-Msi-1-expressing cells in the postnatal and adult CNS. Postnatally, m-Msi-1 was expressed in proliferative neuronal precursors in the external granule cell layer of the cerebellum and in the anterior corner of the subventricular zone of the lateral ventricles. In gliogenesis, the persistent expression of m-Msi-1 was observed in cells of the astrocyte lineage ranging from proliferative glial precursors in the subventricular zone (SVZ), to differentiated astrocytes in the parenchyma. In addition, we showed that m-Mis-1 was still expressed in proliferating cells in the adult SVZ, which may contain neural precursor or stem cells. Another neural RNA-binding protein Hu (the mammalian homolog of Drosophila neuronal RNA-binding protein Elav) was present in postmitotic neurons throughout the development of the CNS, and its pattern of expression was compared with that of m-Msi-1. These observations imply that these two RNA-binding proteins may be involved in the development of neurons and glia by regulating gene expression at the post-transcriptional level.
KW - Astrocyte lineage
KW - Drosophila musashi
KW - Hu
KW - Mouse-Musashi-1 (m-Msi- 1)
KW - Neuronal and glial precursor cells
KW - Postnatal CNS
KW - RNA-binding proteins
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UR - http://www.scopus.com/inward/citedby.url?scp=0030774260&partnerID=8YFLogxK
U2 - 10.1523/jneurosci.17-21-08300.1997
DO - 10.1523/jneurosci.17-21-08300.1997
M3 - Article
C2 - 9334405
AN - SCOPUS:0030774260
VL - 17
SP - 8300
EP - 8312
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 21
ER -