TY - JOUR
T1 - Expression of sialylated Lewis(x) gangliosides in cultured lens epithelial cells from rhesus monkey
AU - Ogiso, Manabu
AU - Takehana, Makoto
AU - Kobayashi, Shizuko
AU - Hoshi, Motonori
PY - 1998/6
Y1 - 1998/6
N2 - Monkey and human lenses contain essentially the same glycosphingolipids, and Lewis(x) and sialylated Lewis(x) epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewis(x) epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewis(x) gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.
AB - Monkey and human lenses contain essentially the same glycosphingolipids, and Lewis(x) and sialylated Lewis(x) epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewis(x) epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewis(x) gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.
KW - Ganglioside
KW - Glycosphingolipid
KW - Lens epithelial cell
KW - Rhesus monkey
KW - Sialyl-Lewis(x)
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U2 - 10.1006/exer.1998.0489
DO - 10.1006/exer.1998.0489
M3 - Article
C2 - 9657909
AN - SCOPUS:0032096368
VL - 66
SP - 765
EP - 773
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 6
ER -