Expression of sialylated Lewis(x) gangliosides in cultured lens epithelial cells from rhesus monkey

Manabu Ogiso, Makoto Takehana, Shizuko Kobayashi, Motonori Hoshi

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Monkey and human lenses contain essentially the same glycosphingolipids, and Lewis(x) and sialylated Lewis(x) epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewis(x) epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewis(x) gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.

本文言語English
ページ(範囲)765-773
ページ数9
ジャーナルExperimental Eye Research
66
6
DOI
出版ステータスPublished - 1998 6

ASJC Scopus subject areas

  • 眼科学
  • 感覚系
  • 細胞および分子神経科学

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