Expression of the adrenomedullin gene in adipose tissue

Takuo Nambu, Hiroshi Arai, Yasato Komatsu, Akihiro Yasoda, Kenji Moriyama, Naotetsu Kanamoto, Hiroshi Itoh, Kazuwa Nakao

研究成果: Article査読

33 被引用数 (Scopus)

抄録

Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5-3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma-vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity.

本文言語English
ページ(範囲)17-22
ページ数6
ジャーナルRegulatory Peptides
132
1-3
DOI
出版ステータスPublished - 2005 12月 15
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 内分泌学
  • 臨床生化学
  • 細胞および分子神経科学

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