Expression of the neural RNA- binding protein Musashi1 in human gliomas

Masahiro Toda, Yukihiko Iizuka, Wangjie Yu, Takao Imai, Eiji Ikeda, Kazunari Yoshida, Takeshi Kawase, Yutaka Kawakami, Hideyuki Okano, Keiichi Uyemura

研究成果: Article査読

137 被引用数 (Scopus)

抄録

Tumor cells arising from a particular tissue may exhibit the same gene expression patterns as their precursor cells. To test this proposition, we have analyzed the expression of a neural RNA-binding protein, Musashi1, in primary human central nervous system (CNS) tumors. In rodents, Musashi1 is expressed predominantly in proliferating multipotent neural precursor cells, but not in newly generated postmitotic neurons. The expression of Musashi1 is downregulated with the successive progression of neurogenesis. In normal adult human tissues, we detected low levels of Musashi1 expression in brain and testis by RT-PCR analysis. In an RNA panel of 32 cancer tissues and cell lines, elevated expression of Musashi1 was seen in all five malignant gliomas studied, in contrast to the slight expression seen in other tumor cells, including those in several melanomas and a prostate cancer. Western blot analysis showed strong Musashi1 expression in malignant gliomas compared with nonneoplastic brain tissue. Glioblastomas, the most malignant form of glioma, showed higher Musashi1 expression than less malignant gliomas by immunohistochemical analysis. Tumors with strong Musashi1 expression tended to have high proliferative activity. Thus, the expression of Musashi1 correlated with the grade of the malignancy and proliferative activity in gliomas. These results suggest that primary CNS tumors may share gene expression patterns with primitive, undifferentiated CNS cells and that Musashi1 may be a useful marker for the diagnosis of CNS tumors.

本文言語English
ページ(範囲)1-7
ページ数7
ジャーナルGlia
34
1
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 神経学
  • 細胞および分子神経科学

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