Expression of type V secretory phospholipase A2 in myocardial remodelling after infarction

Yukio Ishikawa, K. Komiyama, S. Masuda, M. Murakami, Y. Akasaka, K. Ito, Y. Akishima-Fukasawa, M. Kimura, A. Fujimoto, I. Kudo, T. Ishii

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Aims: Secretory phospholipase A2 is associated with ischaemic injury in the human heart, but the distribution of type V secretory phospholipase A2 (sPLA2-V) remains unknown. The significance of sPLA2-V in myocardial infarction was investigated histopathologically. Methods: Sequential changes in the localization of sPLA2-V and its mRNA in myocardial tissues obtained from 30 autopsied hearts were examined by immunohistochemistry and in situ hybridization and compared with those of fibronectin, vascular endothelial growth factor (VEGF), interleukin (IL)-1β, tumour necrosis factor (TNF)-α, and cyclooxygenase-2 (COX-2). Results: No expression of sPLA2-V was observed in normal heart, but it was promptly expressed in wavy myofibres positive for fibronectin just after the onset of infarction. sPLA2-V was subsequently expressed in ischaemic cardiomyocytes around the lesion. The expression decreased at the granulation tissue and disappeared at the chronic stage with scar formation. The distribution of the signal for SPLA2-V mRNA paralleled that of the protein. Ischaemic myocytes around the lesion expressed VEGF, IL-1β, TNF-α and COX-2 at all stages. Conclusions: sPLA2-V production in myocardium is limited to the acute phase of infarction. sPLA2-V may play a dual role, acting both to remove degraded cell-membrane through cooperative activity with COX-2 in necrotic areas and to attack ischaemic myocytes around the lesion via degradation of membrane phospholipids.

本文言語English
ページ(範囲)257-267
ページ数11
ジャーナルHistopathology
47
3
DOI
出版ステータスPublished - 2005 9
外部発表はい

ASJC Scopus subject areas

  • 病理学および法医学
  • 組織学

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