TY - JOUR
T1 - Expression profiling of the mouse early embryo
T2 - Reflections and perspectives
AU - Ko, Minoru S.H.
PY - 2006/9
Y1 - 2006/9
N2 - The laboratory mouse model plays important roles in our understanding of early mammalian development and provides an invaluable model for human early embryos, which are difficult to study for ethical and technical reasons. A comprehensive collection of cDNA clones, their sequences, and complete genome sequence information, which have been accumulated over the past two decades, reveal even further the value of the mouse models. Here, the progress in global gene expression profiling in early mouse embryos and, to some extent, stem cells is reviewed and future directions and challenges are discussed. The discussions include the restatement of global gene expression profiles as a snapshot of cellular status, and subsequent distinction between the differentiation state and physiological state of the cells. The discussions then extend to the biological problems that can be addressed only through global expression profiling, including a bird's-eye view of global gene expression changes, molecular index for developmental potency, cell lineage trajectory, microarray-guided cell manipulation, and the possibility of delineating gene regulatory cascades and networks.
AB - The laboratory mouse model plays important roles in our understanding of early mammalian development and provides an invaluable model for human early embryos, which are difficult to study for ethical and technical reasons. A comprehensive collection of cDNA clones, their sequences, and complete genome sequence information, which have been accumulated over the past two decades, reveal even further the value of the mouse models. Here, the progress in global gene expression profiling in early mouse embryos and, to some extent, stem cells is reviewed and future directions and challenges are discussed. The discussions include the restatement of global gene expression profiles as a snapshot of cellular status, and subsequent distinction between the differentiation state and physiological state of the cells. The discussions then extend to the biological problems that can be addressed only through global expression profiling, including a bird's-eye view of global gene expression changes, molecular index for developmental potency, cell lineage trajectory, microarray-guided cell manipulation, and the possibility of delineating gene regulatory cascades and networks.
KW - Embryogenomics
KW - Expression profiling
KW - Large-scale analysis
KW - Microarray
KW - Preimplantation embryos
KW - Stem cells
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U2 - 10.1002/dvdy.20859
DO - 10.1002/dvdy.20859
M3 - Review article
C2 - 16739220
AN - SCOPUS:33748324824
VL - 235
SP - 2437
EP - 2448
JO - American Journal of Anatomy
JF - American Journal of Anatomy
SN - 1058-8388
IS - 9
ER -