Extension of donor lung preservation with hypothermic storage after normothermic ex vivo lung perfusion

Michael Kuan Yew Hsin, Ilker Iskender, Daisuke Nakajima, Manyin Chen, Hyunhee Kim, Pedro Reck Dos Santos, Jin Sakamoto, Jingu Lee, Kohei Hashimoto, Constantine Harmantas, David Hwang, Tom Waddell, Mingyao Liu, Shaf Keshavjee, Marcelo Cypel

研究成果: Article査読

28 被引用数 (Scopus)


Background Ex vivo lung perfusion (EVLP) allows normothermic evaluation and treatment of donor lungs not currently acceptable for transplant and improves organ use. Donor lungs undergo a period of cold preservation before (cold ischemic time [CIT]-1) and after (CIT-2) EVLP. We investigated the effect of an extended CIT-2 on lung function after transplantation. Methods Explanted pig lungs, preserved in low-potassium dextran flush (Perfadex) at 4°C for 10 hours, were subjected to 6 hours of EVLP. They were subsequently allocated to 2 groups: short CIT-2 (CIT-2 = 2 hours; n = 5), and long CIT-2 (CIT-2 = 10 hours; n = 5). In a control group (n = 6), explanted lungs were placed in cold static preservation for 24 hours without EVLP. After the total preservation period, the left lung was transplanted in all groups. Results After 4 hours of reperfusion, oxygenation function, acute lung injury score, inflammatory markers, and cell death pathway markers were similar between short and long CIT-2 groups. Both EVLP groups fared significantly better than the control group in oxygenation function (p < 0.05). Conclusions The intervention of EVLP improved lung function after transplantation, and this was not affected by a prolonged cold static preservation time after EVLP. These results provide the basis for a practical prolonged lung preservation strategy using a combination of cold and warm preservation techniques, which may improve lung transplantation logistics and outcomes.

ジャーナルJournal of Heart and Lung Transplantation
出版ステータスPublished - 2016 1 1

ASJC Scopus subject areas

  • 外科
  • 呼吸器内科
  • 循環器および心血管医学
  • 移植


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