External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo

Junichi Kumamoto, Makiko Goto, Sumiko Denda, Masashi Nakatani, Yuya Takasugi, Katsunori Tsuchiya, Yuji Shimizu, Yusuke Takatsuru, Mitsuhiro Denda

研究成果: Letter

3 引用 (Scopus)

抜粋

Exocytosis of lamellar bodies at the uppermost nucleated layer of the epidermis is a crucial process for epidermal permeability barrier homoeostasis. We have previously suggested that skin surface electric potential might be associated with barrier homoeostasis. Thus, we hypothesized that the potential might drive exocytosis of lamellar bodies. In this study, we tested this idea by applying negative electric potential (-0.5 V) to human skin samples ex vivo for 2 h and observing the ultrastructure of the uppermost layer. The secretion of lamellar bodies was accelerated in the potential-applied skin, compared to that in untreated control skin. Multiphoton observation indicated that extracellular lipid domains were more extensive in treated skin than in control skin. Moreover, the calcium ion gradient was greater at the uppermost layer of the epidermis of treated skin, compared to that in control skin. These results indicate that electric potential may regulate lamellar body secretion in healthy human skin.

元の言語English
ページ(範囲)421-423
ページ数3
ジャーナルExperimental Dermatology
22
発行部数6
DOI
出版物ステータスPublished - 2013 6 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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    Kumamoto, J., Goto, M., Denda, S., Nakatani, M., Takasugi, Y., Tsuchiya, K., Shimizu, Y., Takatsuru, Y., & Denda, M. (2013). External negative electric potential accelerates exocytosis of lamellar bodies in human skin ex vivo. Experimental Dermatology, 22(6), 421-423. https://doi.org/10.1111/exd.12145