Fabrication of type i collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions

Junghyo Yoon, Jaehoon Kim, Hyo Eun Jeong, Ryo Sudo, Myung Jin Park, Seok Chung

研究成果: Article査読

7 被引用数 (Scopus)

抄録

We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.

本文言語English
論文番号035014
ジャーナルBiofabrication
8
3
DOI
出版ステータスPublished - 2016 8 26

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生化学
  • 生体材料
  • 生体医工学

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